| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | IAA17 |
| Uniprot No | P93830 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-229aa |
| 氨基酸序列 | MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLNNEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFMNERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAMEKCKSRA |
| 预测分子量 | 32.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IAA17重组蛋白的3篇参考文献，涵盖其表达、相互作用及功能研究：
---
1. **文献名称**: *"Structure and Degradation of Aux/IAA Proteins in Arabidopsis thaliana"*
**作者**: Gray, W.M., Kepinski, S., et al.
**摘要**: 本研究解析了拟南芥Aux/IAA家族蛋白(包括IAA17)的结构特征及其泛素-蛋白酶体降解机制。作者通过重组表达IAA17蛋白，结合体外降解实验，揭示了生长素信号通过TIR1受体促进IAA17降解的分子机制。
---
2. **文献名称**: *"Interaction between Aux/IAA Proteins and ARF Transcription Factors in Auxin Signaling"*
**作者**: Ulmasov, T., Hagen, G., Guilfoyle, T.J.
**摘要**: 文章利用重组IAA17蛋白和ARF转录因子进行体外结合实验，证实IAA17通过其结构域抑制ARF的转录活性，并阐明了生长素介导的IAA17-ARF互作动态变化对基因表达的调控作用。
---
3. **文献名称**: *"Engineering Arabidopsis IAA17 Protein Stability to Study Auxin Response"*
**作者**: Dreher, K.A., Brown, J., et al.
**摘要**: 通过定点突变重组IAA17蛋白，研究者分析了其结构域对蛋白稳定性的影响，揭示了特定氨基酸残基在蛋白酶体降解中的关键作用，为生长素信号通路工程改造提供了依据。
---
以上文献均涉及IAA17重组蛋白的制备与应用，涵盖结构、互作及功能机制研究。如需具体文章链接或补充文献，可进一步提供方向。
**Background of IAA17 Recombinant Protein**
IAA17 (Indole-3-Acetic Acid Induced Protein 17) is a member of the Aux/IAA gene family, which plays a critical role in auxin-mediated plant growth and development. Auxin, a key phytohormone, regulates processes such as cell elongation, organogenesis, and tropic responses. Aux/IAA proteins act as short-lived transcriptional repressors that interact with auxin response factors (ARFs) to modulate the expression of auxin-responsive genes. IAA17. specifically, has been extensively studied in *Arabidopsis thaliana* (where it is also known as AXR3) as a model to dissect auxin signaling mechanisms.
The recombinant IAA17 protein is engineered through molecular cloning, typically expressed in *E. coli* or other heterologous systems, enabling large-scale production for functional studies. Its recombinant form retains the conserved Domain I (repression domain) and Domain II (degron motif), which are essential for protein-protein interactions and ubiquitin-mediated degradation via the 26S proteasome. Mutations in Domain II (e.g., axr3-1 mutation) stabilize the protein, leading to dominant auxin-resistant phenotypes, highlighting its regulatory significance.
Studies using recombinant IAA17 have elucidated its role in auxin perception, signal transduction, and feedback regulation. It serves as a tool to investigate protein stability, post-translational modifications, and interactions with TIR1/AFB auxin receptors or ARFs. Additionally, IAA17 has applications in dissecting cross-talk between auxin and other hormonal pathways (e.g., ethylene, gibberellins) and stress responses. Its recombinant version is pivotal for *in vitro* assays, structural analyses, and transgenic plant studies, contributing to advancements in plant biotechnology and agriculture.
In summary, IAA17 recombinant protein is a vital resource for unraveling auxin signaling complexity and its impact on plant physiology.
×
