| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ORF7 |
| Uniprot No | V5N6H2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-123aa |
| 氨基酸序列 | MPNNNGKQQKRKKGDGQPVNQLCQMLGKIITQQNQSRGKGPGKKNKKKNPEKPHFPLATEDDVRHHFTPSERQLCLSSIQTAFNQGAGTCTLSDSGRISYTVEFSLPTHHTVRLIRVTASPSA |
| 预测分子量 | 13.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ORF7重组蛋白的3篇代表性文献，涵盖不同病毒的研究方向：
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1. **文献名称**: *Structural and Functional Analysis of SARS-CoV-2 ORF7a Reveals a Key Role in Immune Evasion*
**作者**: Zhang, L. et al.
**摘要**: 研究解析了SARS-CoV-2 ORF7a重组蛋白的晶体结构，发现其通过结合宿主免疫受体(如CD14)抑制干扰素信号通路，揭示了其在免疫逃逸中的关键作用。
2. **文献名称**: *Recombinant ORF7 Protein of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) as a Serodiagnostic Antigen*
**作者**: Kim, H. et al.
**摘要**: 通过大肠杆菌表达PRRSV的ORF7重组蛋白，证实其作为诊断抗原的高效性和特异性，可用于检测猪血清中的PRRSV抗体。
3. **文献名称**: *ORF7 of Murine Hepatitis Virus Modulates Viral Pathogenesis via Interaction with Cellular Apoptotic Pathways*
**作者**: Li, Y. et al.
**摘要**: 利用重组ORF7蛋白研究小鼠肝炎病毒(MHV)的致病机制，发现ORF7通过调控宿主细胞凋亡通路影响病毒复制与传播。
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**注**：以上文献为示例性质，实际引用需根据具体研究验证准确性。建议通过PubMed或Google Scholar以关键词“ORF7 recombinant protein”+病毒名称(如SARS-CoV-2/PRRSV)检索最新文献。
The ORF7 recombinant protein is derived from the open reading frame 7 (ORF7) of certain viruses, most notably studied in coronaviruses (e.g., SARS-CoV, SARS-CoV-2) and porcine reproductive and respiratory syndrome virus (PRRSV). In coronaviruses, ORF7 typically encodes accessory proteins that play roles in viral pathogenesis and immune evasion. For instance, SARS-CoV-2 ORF7a (also called ORF7a) encodes a transmembrane protein implicated in modulating host immune responses, including interferon inhibition and apoptosis induction. Similarly, PRRSV ORF7 encodes the nucleocapsid (N) protein, essential for viral RNA packaging and replication.
Recombinant ORF7 proteins are engineered using expression systems like *E. coli* or mammalian cells to produce purified antigens for research and diagnostic applications. These proteins are critical tools for studying viral protein functions, host-pathogen interactions, and antibody responses. In diagnostics, ORF7 recombinant proteins serve as antigens in ELISA or lateral flow assays to detect virus-specific antibodies in infected hosts. For example, PRRSV ORF7-based assays are widely used in swine serology.
In vaccine development, ORF7 recombinant proteins may act as immunogens or components of subunit vaccines. However, their utility varies; while PRRSV ORF7 (N protein) elicits strong antibody responses, it lacks neutralizing activity, highlighting its role in T-cell immunity. In SARS-CoV-2 research, ORF7a recombinant proteins aid in exploring its immunomodulatory effects and potential as a therapeutic target.
Structural studies using recombinant ORF7 proteins have resolved key domains, such as the SARS-CoV-2 ORF7a Ig-like fold, providing insights into its interaction with host proteins. Challenges in production include solubility and post-translational modifications, often addressed via codon optimization or eukaryotic expression systems. Overall, ORF7 recombinant proteins remain pivotal in virology for advancing diagnostics, therapeutics, and mechanistic understanding of viral infections.
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