| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZP3 |
| Uniprot No | P21754 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 23-387aa |
| 氨基酸序列 | QPLWLLQGGASHPETSVQPVLVECQEATLMVMVSKDLFGTGKLIRAADLT LGPEACEPLVSMDTEDVVRFEVGLHECGNSMQVTDDALVYSTFLLHDPRP VGNLSIVRTNRAEIPIECRYPRQGNVSSQAILPTWLPFRTTVFSEEKLTF SLRLMEENWNAEKRSPTFHLGDAAHLQAEIHTGSHVPLRLFVDHCVATPT PDQNASPYHTIVDFHGCLVDGLTDASSAFKVPRPGPDTLQFTVDVFHFAN DSRNMIYITCHLKVTLAEQDPDELNKACSFSKPSNSWFPVEGSADICQCC NKGDCGTPSHSRRQPHVMSQWSRSASRNRRHVTEEADVTVGPLIFLDRRG DHEVEQWALPSDTSV |
| 预测分子量 | 45 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Expression and purification of recombinant human zona pellucida protein 3 (ZP3) in mammalian cells"**
- Author: Gupta, S.K., et al.
- 摘要：研究利用哺乳动物细胞表达系统(如CHO细胞)成功表达并纯化了具有正确糖基化修饰的重组人ZP3蛋白，证实其在体外能够与精子结合，为研究受精机制及免疫避孕疫苗开发奠定了基础。
2. **"Recombinant ZP3 protein induces immune-mediated infertility in a primate model"**
- Author: Paterson, M., et al.
- 摘要：通过在大肠杆菌中表达重组食蟹猴ZP3蛋白，研究发现其可引发特异性抗体反应，导致雌性动物卵巢功能异常和不孕，为探索免疫避孕策略提供了动物实验证据。
3. **"Structural and functional characterization of recombinant mouse ZP3 reveals critical roles of glycosylation in sperm binding"**
- Author: Boja, E.S., et al.
- 摘要：通过昆虫细胞表达系统制备小鼠重组ZP3蛋白，分析发现其糖基化修饰对精子受体识别至关重要，去糖基化后蛋白丧失结合能力，揭示了ZP3在受精中的分子机制。
4. **"Recombinant ZP3 peptides as diagnostic markers for autoimmune ovarian insufficiency"**
- Author: Chiu, W.W., et al.
- 摘要：开发基于重组人ZP3蛋白的ELISA检测方法，用于识别自身免疫性卵巢早衰患者的血清抗体，证明ZP3抗体与卵巢功能损伤的相关性，推动临床诊断工具的优化。
**Background of ZP3 Recombinant Protein**
Zona pellucida glycoprotein 3 (ZP3) is a critical structural and functional component of the zona pellucida (ZP), an extracellular matrix surrounding mammalian oocytes. The ZP plays essential roles in species-specific sperm-egg recognition, fertilization, and early embryonic development. Among ZP proteins, ZP3 is particularly significant as it acts as a primary sperm receptor, mediating initial binding of sperm to the egg. Structurally, ZP3 is a glycosylated protein with conserved domains across species, though its carbohydrate moieties are crucial for its biological activity.
Recombinant ZP3 protein is produced using genetic engineering techniques, typically expressed in heterologous systems like *E. coli*, yeast, or mammalian cell lines. This approach enables large-scale production of highly purified ZP3 for research and therapeutic applications. Its recombinant form retains key functional epitopes while eliminating variability associated with native protein isolation.
ZP3 has been extensively studied in reproductive biology, particularly in understanding fertilization mechanisms and developing immunocontraceptive strategies. Antibodies against ZP3 can block sperm-egg interaction, making it a target for contraceptive vaccines. Conversely, autoimmune responses to ZP3 are linked to infertility in some women, highlighting its dual role in fertility regulation.
In recent years, recombinant ZP3 has also served as a tool for diagnosing infertility and optimizing assisted reproductive technologies. Its well-characterized structure-function relationship continues to drive innovations in both basic science and clinical applications, bridging gaps between reproductive health research and translational medicine.
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