| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | YPEL3 |
| Uniprot No | P61236 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-119aa |
| 氨基酸序列 | MVRISKPKTFQAYLDDCHRRYSCAHCRAHLANHDDLISKSFQGSQGRAYLFNSVVNVGCGPAEERVLLTGLHAVADIHCENCKTTLGWKYEQAFESSQKYKEGKYIIELNHMIKDNGWD |
| 预测分子量 | 29.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于典型研究方向的假设参考文献示例(请注意，这些信息为模拟示例，实际文献需通过学术数据库核实)：
1. **标题**: "YPEL3 Recombinant Protein Induces Cellular Senescence via p53 Pathway"
**作者**: Zhang L, et al.
**摘要**: 研究利用大肠杆菌表达系统制备YPEL3重组蛋白，证实其通过激活p53信号通路诱导人成纤维细胞衰老，为衰老相关疾病研究提供工具。
2. **标题**: "Expression and Functional Analysis of YPEL3 in Colorectal Cancer"
**作者**: Kim S, Park JH.
**摘要**: 通过哺乳动物细胞表达YPEL3重组蛋白，发现其抑制结直肠癌细胞增殖并促进凋亡，提示其作为肿瘤抑制因子的潜在作用。
3. **标题**: "Purification and Characterization of YPEL3 Recombinant Protein in Insect Cells"
**作者**: Gupta R, et al.
**摘要**: 采用杆状病毒-昆虫细胞系统高效表达并纯化YPEL3蛋白，验证其与HSP90的相互作用，为分子机制研究奠定基础。
4. **标题**: "YPEL3 Recombinant Protein Attenuates Cardiac Hypertrophy in vitro"
**作者**: Chen X, et al.
**摘要**: 在心肌细胞模型中，YPEL3重组蛋白通过抑制AKT/mTOR通路减轻病理性心肌肥大，提示治疗心脏疾病的潜力。
**建议**：使用PubMed、Web of Science等平台，以“YPEL3 recombinant protein”或“YPEL3 expression”为关键词检索最新文献，获取真实数据。
YPEL3 (Yippee-like 3) is a member of the evolutionarily conserved YPEL protein family, initially identified through homology to the Drosophila Yippee gene implicated in cell cycle regulation. This nuclear-localized protein, encoded by the YPEL3 gene on human chromosome 16q22.1. consists of 125 amino acids and contains a conserved Yippee-type zinc-binding domain. Studies suggest its involvement in cellular senescence, proliferation suppression, and tumorigenesis. YPEL3 expression is tightly regulated during cell cycle progression, peaking at the G1/S phase transition. It interacts with key cell cycle regulators, including p53. to induce cell cycle arrest through p21 activation.
In cancer biology, YPEL3 exhibits dual roles: it acts as a tumor suppressor in multiple cancers (e.g., hepatocellular carcinoma, cervical cancer) where its downregulation correlates with poor prognosis, while paradoxically promoting metastasis in some contexts. This functional complexity may stem from tissue-specific signaling networks. Recombinant YPEL3 protein, typically produced in Escherichia coli or mammalian expression systems with purification tags (e.g., His-tag), enables functional studies of its biochemical properties, protein interactions, and therapeutic potential. Current research focuses on elucidating its role in senescence-associated pathways, DNA damage response, and epithelial-mesenchymal transition, with implications for cancer therapy and regenerative medicine.
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