| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | YPEL1 |
| Uniprot No | O60688 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-119aa |
| 氨基酸序列 | MVKMTKSKTFQAYLPNCHRTYSCIHCRAHLANHDELISKSFQGSQGRAYLFNSVVNVGCGPAEERVLLTGLHAVADIYCENCKTTLGWKYEHAFESSQKYKEGKFIIELAHMIKDNGWE |
| 预测分子量 | 29.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于YPEL1重组蛋白的3篇代表性文献(示例为模拟内容，实际文献需通过学术数据库查询)：
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1. **文献名称**：*YPEL1 induces cellular senescence by regulating mitochondrial dynamics in cancer cells*
**作者**：Smith A, et al.
**摘要**：本研究通过重组YPEL1蛋白体外表达，发现其通过激活Drp1依赖性线粒体分裂，诱导线粒体功能障碍，从而促进肿瘤细胞衰老，揭示了YPEL1作为抑癌基因的新机制。
2. **文献名称**：*Recombinant YPEL1 protein suppresses lung adenocarcinoma progression via Hippo-YAP pathway*
**作者**：Chen L, et al.
**摘要**：利用大肠杆菌表达系统纯化YPEL1重组蛋白，证实其通过抑制YAP核转位阻断Hippo通路，抑制肺癌细胞增殖及迁移，为靶向治疗提供潜在策略。
3. **文献名称**：*Structural and functional characterization of human YPEL1 recombinant protein*
**作者**：Wang X, et al.
**摘要**：首次解析人源YPEL1重组蛋白的晶体结构，结合功能实验发现其N端结构域介导与14-3-3蛋白互作，调控细胞周期G1/S期阻滞，为分子机制研究奠定基础。
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**提示**：实际文献可通过PubMed、Google Scholar等平台以关键词“YPEL1 recombinant protein”或“YPEL1 function”检索，建议关注其参与细胞周期、凋亡及肿瘤抑制的研究方向。
YPEL1 (Yippee-like 1) is a conserved eukaryotic protein belonging to the Yippee family, initially identified through its homology to the *Drosophila* Yippee gene. This family is characterized by a conserved "Yippee" domain, implicated in metal ion binding and protein-protein interactions. YPEL1 is ubiquitously expressed in human tissues and localized to the nucleus and cytoplasm. It plays a multifaceted role in cellular processes, including cell cycle regulation, proliferation, senescence, and apoptosis. Studies suggest YPEL1 acts as a stress-responsive protein, with its expression modulated by DNA damage, oxidative stress, and oncogenic signaling pathways.
Recombinant YPEL1 protein is engineered for in vitro studies to dissect its molecular mechanisms. Typically produced in *E. coli* or mammalian expression systems, it retains functional domains critical for binding partners like cyclin-dependent kinases (CDKs) or transcription factors. The recombinant form often includes affinity tags (e.g., His-tag) for purification and detection. Research utilizing YPEL1 recombinant protein has highlighted its dual role in cancer biology—acting as a tumor suppressor in some contexts (e.g., inhibiting epithelial-mesenchymal transition) or a potential oncogene in others, depending on cellular context and post-translational modifications.
Current applications span cancer therapeutics, aging research, and regenerative medicine. For example, YPEL1 overexpression induces senescence in cancer cells, suggesting therapeutic potential. Conversely, its downregulation is linked to tissue repair processes. Despite progress, YPEL1's precise molecular targets and regulatory networks remain under investigation, necessitating further structural and functional studies using recombinant protein tools.
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