| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Veph1 |
| Uniprot No | Q14D04 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-833aa |
| 氨基酸序列 | MHQLFRLVLGQKDLSRAGDLFSLDDSEIEDSLTEALEQIKIISSSSDYQTNNNDQAVVEICITRITTAIRETESIEKHAKALVGLWDSCLEHNLRPFGKDEDTPHAKIASDIMSCILQNYNRPPVMALAIPIAVKFLHRGNKELCRNMSNYLSLAAITKADLLADHTEVIVKSILQGNTMLLRVLPAVYEKQPQPINRHLTELLALMSQLEQPEQYHLLRLLHVAAKKKQLEVVQKCIPFLIGHLKDSTHNDIILNILIEIAVYEPVALNSFLPMLKEIGERFPYLTGQMARIYGAVGHVDEERARSCLTYLVSQLANMEHSFHHILLLEIKSITDTFSSILGPQSRDIFRMSNSFTAIAKLLTRQLENTKAGSGRRKISTEIEFPEKLEETKLIVTENEDHEKLQVKIQAFEDKINAGSNTPGSIRRYSLGQVSKEERKNIRFNRSKSLAFHTMLTKGVGSDDGEDENRGDIPASISLSEIDPLGQGNDKLPFKTDTERSQLGESSVSYPNIIHIDSENLSETVKENSQEETPETTASPIEYQDKLYLHLKKNLSKVKAYAMEIGKKIPVPDQCTIEDTVRSCVAKLFFTCSLKGHYCLYSKSSFILISQEPQPWIQIMFLFQQSLFPEPLSIQSHSVQFLRALWEKTQAGGAHSFETAMMESTFPQQKDLDQVQLHLEEVRFFDVFGFSETAGAWQCFMCNNPEKATVVNQDGQPLIEGKLKEKQVRWKFIKRWKTRYFTLAGNQLLFQKGKSKDDPDDCPIELSKVQSVKAVAKKRRDRSLPRAFEIFTDNKTYVFKAKDEKNAEEWLQCINVAVAQAKERESREVTTYL |
| 预测分子量 | 94,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于VEPH1重组蛋白的3篇代表性参考文献示例(注：以下内容为模拟虚构，实际文献需通过学术数据库检索确认)：
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1. **文献名称**: *VEPH1 interacts with the Hippo pathway to suppress ovarian cancer progression*
**作者**: Smith, J.R., et al.
**摘要**: 本研究利用重组VEPH1蛋白探究其在卵巢癌细胞中的功能。实验发现，重组VEPH1过表达通过抑制Hippo信号通路下游效应蛋白YAP/TAZ的核定位，显著降低肿瘤细胞增殖和迁移能力，提示VEPH1可能作为肿瘤抑制因子发挥作用。
2. **文献名称**: *Structural and functional analysis of VEPH1 in renal tubular development*
**作者**: Tanaka, M., et al.
**摘要**: 通过大肠杆菌表达系统纯化VEPH1重组蛋白，结合X射线晶体学解析其N端结构域的三维构象。功能实验表明，重组VEPH1可增强细胞间粘附连接蛋白(如E-cadherin)的稳定性，进而调控肾脏上皮细胞分化。
3. **文献名称**: *VEPH1 recombinant protein attenuates TGF-β-induced fibrosis in cardiac fibroblasts*
**作者**: Chen, L., et al.
**摘要**: 研究证实，外源性添加重组VEPH1蛋白能阻断TGF-β/Smad3信号通路的激活，抑制心脏成纤维细胞的胶原沉积和肌成纤维细胞转化，为心肌纤维化治疗提供潜在靶点。
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**备注**：真实文献需通过PubMed、Web of Science等平台以“VEPH1 recombinant protein”或“VEPH1 AND (expression/purification/function)”为关键词检索。若研究较少，可扩展至VEPH1相关信号通路(如Hippo、TGF-β)或疾病模型的研究。
Veph1 (Ventral Epidermis and Heart Development Protein 1) is a conserved eukaryotic protein implicated in developmental regulation and cellular signaling. Initially identified through genetic studies in model organisms, it has been linked to embryonic patterning, particularly in neural tube closure and organogenesis. Veph1 interacts with components of the Hedgehog and Wnt signaling pathways, suggesting its role in coordinating morphogenetic cues. Structurally, it contains a unique N-terminal domain with predicted lipid-binding motifs and a C-terminal region involved in protein-protein interactions.
Recombinant Veph1 protein is engineered for functional studies, typically expressed in *E. coli* or mammalian systems with affinity tags (e.g., His-tag) for purification. Its production enables biochemical characterization, including binding assays to map interaction networks, and cellular studies to assess effects on proliferation/differentiation. Notably, Veph1 dysregulation has been associated with congenital disorders (e.g., neural tube defects) and cancers, making the recombinant protein valuable for investigating disease mechanisms. Recent work highlights its potential as a modulator of autophagy and ciliogenesis, expanding its relevance to neurodegenerative conditions. Current research focuses on resolving its 3D structure and therapeutic targeting possibilities. The recombinant tool thus bridges developmental biology and translational medicine, offering insights into fundamental cell signaling processes.
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