| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Tfap2a |
| Uniprot No | P05549 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-437aa |
| 氨基酸序列 | MLWKLTDNIK YEDCEDRHDG TSNGTARLPQ LGTVGQSPYT SAPPLSHTPN ADFQPPYFPP PYQPIYPQSQ DPYSHVNDPY SLNPLHAQPQ PQHPGWPGQR QSQESGLLHT HRGLPHQLSG LDPRRDYRRH EDLLHGPHAL SSGLGDLSIH SLPHAIEEVP HVEDPGINIP DQTVIKKGPV SLSKSNSNAV SAIPINKDNL FGGVVNPNEV FCSVPGRLSL LSSTSKYKVT VAEVQRRLSP PECLNASLLG GVLRRAKSKN GGRSLREKLD KIGLNLPAGR RKAANVTLLT SLVEGEAVHL ARDFGYVCET EFPAKAVAEF LNRQHSDPNE QVTRKNMLLA TKQICKEFTD LLAQDRSPLG NSRPNPILEP GIQSCLTHFN LISHGFGSPA VCAAVTALQN YLTEALKAMD KMYLSNNPNS HTDNNAKSSD KEEKHRK |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Tfap2a重组蛋白的3篇示例参考文献(注：以下内容为基于领域知识的合理示例，非真实存在的文献)：
1. **文献名称**：*Functional characterization of recombinant Tfap2a in neural crest development*
**作者**：Zhang Y. et al.
**摘要**：本研究通过大肠杆菌表达系统纯化Tfap2a重组蛋白，并证明其能够结合靶基因启动子区域，调控神经嵴细胞迁移和分化。
2. **文献名称**：*Structural analysis of recombinant AP-2α protein reveals dimerization-dependent DNA binding*
**作者**：Garcia R. et al.
**摘要**：利用X射线晶体学解析Tfap2a重组蛋白的三维结构，阐明其二聚化机制及DNA结合域的关键氨基酸残基，为突变导致的面部发育异常提供分子基础。
3. **文献名称**：*Recombinant Tfap2a rescues craniofacial defects in zebrafish embryos*
**作者**：Parker S. et al.
**摘要**：在Tfap2a基因敲除斑马鱼模型中，注射重组Tfap2a蛋白可部分恢复颅面软骨发育，证实其在胚胎形态发生中的直接作用。
(示例仅作参考，实际文献需通过学术数据库检索。)
**Background of Tfap2a Recombinant Protein**
Transcription factor AP-2 alpha (Tfap2a), a member of the AP-2 family of DNA-binding proteins, plays critical roles in embryonic development and cellular differentiation. It regulates gene expression by binding to specific DNA sequences, influencing processes such as cell proliferation, apoptosis, and tissue morphogenesis. Tfap2a is particularly vital in neural crest cell development, craniofacial formation, and organogenesis, with mutations linked to human disorders like branchio-oculo-facial syndrome.
Recombinant Tfap2a protein is engineered using expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, functional protein for research. Its production typically involves cloning the *TFAP2A* gene into expression vectors, followed by affinity chromatography (e.g., His-tag purification) to ensure high purity. The recombinant protein retains the ability to form dimers and bind DNA via its conserved basic helix-span-helix and dimerization domains.
In research, recombinant Tfap2a is used to study transcriptional mechanisms, protein-DNA interactions (e.g., EMSA, ChIP), and signaling pathways in development or disease models. It also serves as a tool to investigate Tfap2a's dual role in cancer—acting as a tumor suppressor or oncogene, depending on context. Additionally, it aids in exploring therapeutic targets for developmental disorders or malignancies linked to AP-2 family dysregulation. The availability of recombinant Tfap2a accelerates functional studies, enabling precise dissection of its regulatory networks in health and disease.
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