| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TESC |
| Uniprot No | Q96BS2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-214aa |
| 氨基酸序列 | GAAHSASEEVRELEGKTGFSSDQIEQLHRRFKQLSGDQPTIRKENFNNVPDLELNPIRSKIVRAFFDNRNLRKGPSGLADEINFEDFLTIMSYFRPIDTTMDEEQVELSRKEKLRFLFHMYDSDSDGRITLEEYRNVVEELLSGNPHIEKESARSIADGAMMEAASVCMGQMEPDQVYEGITFEDFLKIWQGIDIETKMHVRFLNMETMALCH |
| 预测分子量 | 40.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TESC(Tescalcin)重组蛋白的模拟参考文献示例(仅供参考，建议通过学术数据库查询真实文献)：
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1. **文献名称**：*Tescalcin重组蛋白通过调控Wnt信号通路抑制结肠癌细胞增殖*
**作者**：Zhang Y, et al.
**摘要**：研究利用重组Tescalcin蛋白处理结肠癌细胞，发现其通过抑制Wnt/β-catenin通路降低癌细胞增殖和迁移能力，提示TESC在肿瘤治疗中的潜在作用。
2. **文献名称**：*重组Tescalcin在心脏发育中的功能：促进心肌细胞分化*
**作者**：Smith JL, et al.
**摘要**：通过体外表达重组Tescalcin蛋白，证明其能结合ERK信号分子，增强胚胎干细胞向心肌细胞的分化，为心脏再生医学提供新靶点。
3. **文献名称**：*Tescalcin重组蛋白的钙依赖性结构特征分析*
**作者**：Yamamoto K, et al.
**摘要**：利用X射线晶体学解析重组Tescalcin的三维结构，揭示其钙离子结合位点及构象变化机制，为研究其分子互作提供结构基础。
4. **文献名称**：*大肠杆菌中Tescalcin重组蛋白的高效表达与纯化策略*
**作者**：Patel R, et al.
**摘要**：优化了TESC基因在大肠杆菌中的表达条件，开发出基于亲和层析的高纯度重组蛋白制备方案，显著提升蛋白产量及稳定性。
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**注意**：以上内容为模拟生成，实际文献需通过PubMed、Web of Science或Google Scholar等平台检索关键词“Tescalcin recombinant protein”或“TESC recombinant”获取。
**Background of TESC Recombinant Protein**
Tescalcin (TESC), encoded by the *TESC* gene located on human chromosome 13q12.3. is a calcium-binding protein implicated in cellular processes such as differentiation, proliferation, and apoptosis. It belongs to the calcinerin homologous protein (CHP) family and regulates ion transport and signaling pathways, including the MAPK/ERK cascade. TESC interacts with sodium-hydrogen exchangers (NHEs) and other molecular targets, modulating intracellular pH and calcium dynamics, which are critical for cellular homeostasis.
Recombinant TESC protein is engineered using expression systems like *E. coli* or mammalian cells, enabling large-scale production for research and therapeutic applications. Its recombinant form retains functional properties, allowing studies on its role in diseases, particularly cancer, where TESC overexpression is linked to tumor progression and drug resistance. Additionally, TESC is studied in cardiovascular and neurological contexts due to its influence on cell survival and stress responses.
The development of TESC recombinant protein has advanced mechanistic insights into its dual roles as an oncogene or tumor suppressor, depending on cellular context. It also serves as a tool for screening potential therapeutic agents targeting TESC-associated pathways. Challenges in production include maintaining post-translational modifications and solubility, which are addressed through optimized expression and purification strategies. Overall, TESC recombinant protein remains a vital resource for deciphering its biological functions and translational potential.
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