| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBX18 |
| Uniprot No | O95935 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-607aa |
| 氨基酸序列 | MAEKRRGSPCSMLSLKAHAFSVEALIGAEKQQQLQKKRRKLGAEEAAGAVDDGGCSRGGGAGEKGSSEGDEGAALPPPAGATSGPARSGADLERGAAGGCEDGFQQGASPLASPGGSPKGSPARSLARPGTPLPSPQAPRVDLQGAELWKRFHEIGTEMIITKAGRRMFPAMRVKISGLDPHQQYYIAMDIVPVDNKRYRYVYHSSKWMVAGNADSPVPPRVYIHPDSPASGETWMRQVISFDKLKLTNNELDDQGHIILHSMHKYQPRVHVIRKDCGDDLSPIKPVPSGEGVKAFSFPETVFTTVTAYQNQQITRLKIDRNPFAKGFRDSGRNRMGLEALVESYAFWRPSLRTLTFEDIPGIPKQGNASSSTLLQGTGNGVPATHPHLLSGSSCSSPAFHLGPNTSQLCSLAPADYSACARSGLTLNRYSTSLAETYNRLTNQAGETFAPPRTPSYVGVSSSTSVNMSMGGTDGDTFSCPQTSLSMQISGMSPQLQYIMPSPSSNAFATNQTHQGSYNTFRLHSPCALYGYNFSTSPKLAASPEKIVSSQGSFLGSSPSGTMTDRQMLPPVEGVHLLSSGGQQSFFDSRTLGSLTLSSSQVSAHMV |
| 预测分子量 | 80.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **TBX18重组蛋白** 的3篇示例参考文献(内容为模拟，仅供参考)：
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1. **文献名称**: *TBX18 Regulates Sinoatrial Node Development Through Transcriptional Activation of Pacemaker Program*
**作者**: Wiese C, Grieskamp T, et al.
**摘要**: 研究揭示了TBX18重组蛋白在心脏起搏细胞分化中的关键作用，通过体外重组蛋白表达实验证实其直接激活起搏相关基因(如HCN4)，为心律失常治疗提供分子机制依据。
2. **文献名称**: *Recombinant TBX18 Protein Enhances Reprogramming of Cardiac Fibroblasts to Pacemaker-like Cells*
**作者**: Kapoor N, Liang D, et al.
**摘要**: 利用TBX18重组蛋白联合基因转导技术，成功将心脏成纤维细胞重编程为起搏样细胞，证明了其在生物起搏器构建中的潜在应用价值。
3. **文献名称**: *Structural and Functional Analysis of TBX18 in Kidney Ureteric Bud Morphogenesis*
**作者**: Bohnenpoll T, Weiss AC, et al.
**摘要**: 通过重组TBX18蛋白的晶体结构解析，阐明了其T-box结构域与DNA结合的分子机制，并验证了其在肾脏输尿管芽发育中的调控功能。
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**注意**：以上文献为示例，实际研究中请通过 **PubMed/Google Scholar** 检索真实文献(关键词：TBX18 recombinant protein, cardiac development, gene regulation)。
TBX18 recombinant protein is a biologically engineered molecule derived from the T-box transcription factor TBX18. which plays critical roles in embryonic development and tissue specification. TBX18 belongs to the T-box gene family, a group of evolutionarily conserved regulators involved in morphogenesis, cell fate determination, and organogenesis. Specifically, TBX18 is essential for the development of the cardiac conduction system, kidneys, and skeletal structures, with documented functions in sinoatrial node formation, ureteric bud branching, and vertebral patterning.
The recombinant form is typically produced in heterologous expression systems (e.g., E. coli or mammalian cell lines) using DNA sequences encoding the functional domain of human TBX18. This engineered protein retains the DNA-binding capacity of native TBX18. enabling researchers to study its regulatory mechanisms in vitro. Purification processes often incorporate affinity tags (e.g., His-tag) for efficient isolation while maintaining structural integrity.
In research, TBX18 recombinant protein serves as a tool molecule for investigating cellular reprogramming, particularly in generating induced pacemaker cells from cardiomyocytes. It has shown potential in cardiac regeneration studies, where transient TBX18 expression can convert working myocardial cells into pacemaker-like cells. Additionally, it aids in modeling developmental disorders linked to TBX18 mutations, such as congenital heart defects and skeletal malformations. Recent applications extend to tissue engineering and drug screening platforms targeting rhythm disorders.
Studies utilizing this recombinant protein have clarified TBX18's role in suppressing alternative cell lineages during differentiation, highlighting its therapeutic potential in regenerative medicine. Quality assessments typically include SDS-PAGE, Western blotting, and functional assays to confirm DNA-binding activity.
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