| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SPT15 |
| Uniprot No | P13393 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-240aa |
| 氨基酸序列 | ADEERLKEFKEANKIVFDPNTRQVWENQNRDGTKPATTFQSEEDIKRAAPESEKDTSATSGIVPTLQNIVATVTLGCRLDLKTVALHARNAEYNPKRFAAVIMRIREPKTTALIFASGKMVVTGAKSEDDSKLASRKYARIIQKIGFAAKFTDFKIQNIVGSCDVKFPIRLEGLAFSHGTFSSYEPELFPGLIYRMVKPKIVLLIFVSGKIVLTGAKQREEIYQAFEAIYPVLSEFRKM |
| 预测分子量 | 28.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPT15重组蛋白的3篇参考文献示例(文献信息为模拟内容，供参考)：
1. **"Cloning and expression of the yeast SPT15 gene encoding TATA-binding protein"**
- **作者**: Struhl, K. et al.
- **摘要**: 报道了酵母SPT15基因的克隆及在大肠杆菌中的重组表达，分析了TBP(TATA结合蛋白)在基础转录机制中的作用，并验证了重组蛋白的DNA结合活性。
2. **"Crystal structure of recombinant yeast TBP at 1.8 Å resolution"**
- **作者**: Nikolov, D.B. et al.
- **摘要**: 通过X射线晶体学解析了重组酵母SPT15/TBP蛋白的三维结构，揭示了其DNA结合域的关键氨基酸残基，为研究真核转录起始机制提供了结构基础。
3. **"Functional analysis of SPT15 mutations in transcriptional regulation"**
- **作者**: Hahn, S. et al.
- **摘要**: 利用重组SPT15蛋白构建多种突变体，通过体外转录实验证明其C端结构域对RNA聚合酶II招募的调控作用，揭示了TBP在基因特异性表达中的功能。
注：以上文献为示例，实际引用时请核实真实文献信息(可通过PubMed或Google Scholar搜索关键词如“SPT15 recombinant protein”或“yeast TBP expression”获取)。
**Background of SPT15 Recombinant Protein**
SPT15 is a gene-encoding transcription factor originally identified in *Saccharomyces cerevisiae* (baker’s yeast). The protein product, SPT15 (also known as TATA-binding protein or TBP), plays a central role in transcriptional initiation by RNA polymerase II. As a core component of the TFIID complex, SPT15 binds specifically to the TATA box, a conserved DNA sequence motif in eukaryotic gene promoters, facilitating the assembly of the pre-initiation complex (PIC) required for transcription. Its structural and functional conservation across eukaryotes underscores its fundamental role in gene regulation.
Recombinant SPT15 protein is produced through genetic engineering, often expressed in *E. coli* or other heterologous systems for research applications. The recombinant form is typically purified to high homogeneity, enabling detailed biochemical and structural studies. Key applications include *in vitro* transcription assays, promoter-binding studies, and investigations into transcriptional regulation mechanisms. SPT15’s ability to recruit other transcription factors and coactivators makes it a critical tool for dissecting the molecular architecture of transcriptional machinery.
Mutations in SPT15 have been linked to transcriptional dysregulation, providing insights into diseases associated with faulty gene expression. Recombinant SPT15 variants are also used to study the effects of specific mutations on DNA binding or protein-protein interactions. Additionally, its role in yeast models has aided in understanding conserved regulatory pathways in higher eukaryotes.
In biotechnology, SPT15 recombinant protein is utilized for engineering synthetic promoters or optimizing gene expression systems. Its stability and functional versatility make it valuable for both academic research and industrial applications, such as drug screening targeting transcription factors. Overall, SPT15 remains a cornerstone protein for exploring the mechanics of gene expression and developing therapeutic strategies.
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