| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SUPT3H |
| Uniprot No | O75486 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-328aa |
| 氨基酸序列 | MVVFGTTFFNFDSFIGKTDSIEIIGKSVFPYCYHNILPLLNDSGRYSLGDARRPLHETAVLVEDVVHTQLINLLQQAAEVSQLRGARVITPEDLLFLMRKDKKKLRRLLKYMFIRDYKSKIVKGIDEDDLLEDKLSGSNNANKRQKIAQDFLNSIDQTGELLAMFEDDEIDEVKQERMERAERQTRIMDSAQYAEFCESRQLSFSKKASKFRDWLDCSSMEIKPNVVAMEILAYLAYETVAQLVDLALLVRQDMVTKAGDPFSHAISATFIQYHNSAESTAACGVEAHSDAIQPCHIREAIRRYSHRIGPLSPFTNAYRRNGMAFLAC |
| 预测分子量 | 53.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SUPT3H重组蛋白的模拟参考文献示例(基于公开知识整理，非真实文献)：
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1. **文献名称**: *SUPT3H重组蛋白在转录复合物SAGA中的功能解析*
**作者**: Lee, J. et al.
**摘要**: 研究利用重组SUPT3H蛋白揭示了其在SAGA复合体中介导组蛋白乙酰转移酶活性的关键作用，证明其缺失会导致靶基因转录抑制。
2. **文献名称**: *重组人SUPT3H蛋白的体外表达与纯化*
**作者**: Chen, R. & Wang, M.
**摘要**: 报道了一种高效的大肠杆菌表达系统制备重组SUPT3H蛋白的方法，并通过质谱验证其结构完整性，为后续功能研究提供工具。
3. **文献名称**: *SUPT3H蛋白结构域对其染色质重塑活性的影响*
**作者**: Gonzalez, S. et al.
**摘要**: 通过重组蛋白截断体实验，发现SUPT3H的N端结构域是结合SAGA复合体和调控基因启动子区开放性的必需区域。
4. **文献名称**: *SUPT3H重组蛋白与癌症相关突变的关联研究*
**作者**: Kim, T. et al.
**摘要**: 利用重组SUPT3H蛋白模拟体细胞突变，发现特定位点突变会破坏其与TFIID的相互作用，可能参与肿瘤发生中的转录失调。
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**说明**：以上文献为基于SUPT3H已知生物学功能(如参与SAGA复合体、调控转录和染色质修饰)的模拟示例，实际研究请参考PubMed或Web of Science数据库的最新文献。
**Background of SUPT3H Recombinant Protein**
SUPT3H (Suppressor of Ty 3 homolog), also known as SPT3. is a conserved eukaryotic protein that plays a critical role in transcriptional regulation as part of the multi-subunit SPT-ADA-GCN5 acetyltransferase (SAGA) complex. This chromatin-modifying complex is essential for histone acetylation, a key epigenetic mechanism that loosens chromatin structure to facilitate transcriptional activation. SUPT3H directly interacts with the TATA-binding protein (TBP) and other SAGA components, aiding in the recruitment of the complex to gene promoters and regulating RNA polymerase II-dependent transcription.
The recombinant SUPT3H protein is typically produced using expression systems like *E. coli* or mammalian cell cultures, followed by affinity purification to ensure high purity and functionality. Its recombinant form retains critical structural domains, including the TBP-binding region and motifs required for SAGA complex assembly, enabling in vitro studies on chromatin remodeling, transcriptional initiation, and protein-protein interactions.
Research highlights SUPT3H's involvement in cellular processes such as cell cycle progression, DNA repair, and stress response. Dysregulation of SUPT3H has been linked to cancers, neurodegenerative disorders, and developmental defects, underscoring its therapeutic potential. For example, aberrant SUPT3H expression may promote oncogenesis by altering transcription of growth-related genes. Recombinant SUPT3H serves as a vital tool for dissecting SAGA complex mechanisms, screening epigenetic drug candidates, and modeling diseases linked to transcriptional dysregulation. Its application spans molecular biology, drug discovery, and functional genomics.
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