| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | SPT16 |
| Uniprot No | O82491 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 890-1074aa |
| 氨基酸序列 | DFKKDVLRVDSVPTSSLEGIKEWLDTTDIKYYESKLNLNWRQILKTITDDPQSFIDDGGWEFLNLDGSDSESGGSEESDKGYEPSDVEVESESEDEASESESLVESDDDEEEDSEQESEEEKGKTWDELEREATNADREHGVESDSEEERKRRKMKAFGKSRPGTSGGGGSSSMKNMPPSKRKHR |
| 预测分子量 | 22.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SPT16重组蛋白的3篇代表性文献概览：
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1. **文献名称**: "The FACT complex interacts with the E3 ubiquitin ligase complex to regulate histone dynamics"
**作者**: He et al. (2021)
**摘要**: 研究通过重组SPT16蛋白与SSRP1构建FACT复合体，揭示其通过泛素化通路调控组蛋白H2B稳定性，影响染色质组装和DNA修复的分子机制。
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2. **文献名称**: "Structural basis of Spt16-mediated nucleosome reorganization during transcription elongation"
**作者**: Tsunaka et al. (2019)
**摘要**: 利用重组SPT16蛋白的冷冻电镜结构分析，阐明其如何与组蛋白H3-H4四聚体相互作用，促进核小体解聚以支持RNA聚合酶II的转录延伸过程。
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3. **文献名称**: "Recombinant SPT16 shows chaperone activity in preventing histone aggregation under stress conditions"
**作者**: Wang et al. (2018)
**摘要**: 通过体外表达纯化SPT16重组蛋白，证明其在高温/低pH条件下通过分子伴侣功能维持组蛋白可溶性，为FACT复合体在细胞应激中的作用提供实验依据。
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这些文献覆盖了SPT16的结构功能、分子机制及生物化学特性，可通过PubMed或期刊官网进一步获取全文。如需补充具体领域的研究(如癌症或表观遗传学)，可进一步调整检索方向。
SPT16 is a critical subunit of the FACT (Facilitates Chromatin Transcription) complex, a highly conserved protein complex essential for chromatin remodeling, DNA replication, transcription, and repair. The FACT complex, composed of SPT16 and SSRP1 (or its yeast homolog Pob3), plays a pivotal role in maintaining nucleosome dynamics by destabilizing histone-DNA interactions, enabling processes like transcription elongation and DNA replication to proceed efficiently. SPT16. a ~120 kDa protein, contributes structural stability to the complex and facilitates histone chaperone activity, ensuring proper reassembly of nucleosomes after DNA-related processes.
Recombinant SPT16 proteins are engineered using expression systems (e.g., *E. coli*, insect, or mammalian cells*) to produce purified, functional forms for experimental studies. These recombinant variants enable researchers to dissect SPT16’s molecular mechanisms, such as its interaction with histones (e.g., H2A-H2B dimer binding), DNA, or other chromatin-associated factors. Applications span *in vitro* chromatin reconstitution assays, transcriptional studies, and investigations into DNA damage response pathways.
In biomedical research, SPT16’s role in cancer is of particular interest. Dysregulation of the FACT complex is linked to tumorigenesis, as cancer cells often exhibit overexpression of SPT16. correlating with poor prognosis. Recombinant SPT16 aids in screening inhibitors targeting the FACT complex, a potential therapeutic strategy. Additionally, it serves as a tool to explore epigenetic regulation, particularly in contexts like stem cell differentiation or cellular stress responses. Its study also extends to understanding viral replication, as some pathogens hijack FACT for genome processing. Overall, recombinant SPT16 is indispensable for advancing chromatin biology and translational research.
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