| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SERS |
| Uniprot No | P49591 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-233aa |
| 氨基酸序列 | VLDLDLFRVDKGGDPALIRETQEKRFKDPGLVDQLVKADSEWRRCRFRADNLNKLKNLCSKTIGEKMKKKEPVGDDESVPENVLSFDDLTADALANLKVSQIKKVRLLIDEAILKCDAERIKLEAERFENLREIGNLLHPSVPISNDEDVDNKVERIWGDCTVRKKYSHVDLVVMVDGFEGEKGAVVAGSRGYFLKGVLVFLEQALIQYALRTLGSRGYIPIYTPFFMRKEV |
| 预测分子量 | 53.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SERS(表面增强拉曼散射)与重组蛋白结合的3篇代表性文献示例(注：以下内容为模拟生成，实际文献需通过学术数据库核实)：
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1. **文献名称**：*SERS-based biosensor using engineered recombinant proteins for ultrasensitive detection of SARS-CoV-2*
**作者**：Chen, Y. et al.
**摘要**：开发了一种基于重组刺突蛋白修饰的金纳米结构SERS传感器，通过靶向病毒RNA结合域，实现了对新冠病毒的超灵敏检测，检测限达飞摩尔级别。
2. **文献名称**：*Recombinant protein-functionalized nanoparticles for SERS monitoring of protein conformational changes*
**作者**：Wang, L. & Smith, J.
**摘要**：利用基因工程重组蛋白包被银纳米粒子，构建SERS探针，实时监测热诱导下蛋白质构象变化，为药物-蛋白相互作用研究提供新方法。
3. **文献名称**：*Multiplex SERS detection of cancer biomarkers via engineered antibody-fusion proteins*
**作者**：Gupta, R. et al.
**摘要**：设计重组抗体-荧光蛋白融合分子，结合编码SERS纳米标签，实现血清中多种肿瘤标志物的同步高特异性检测，提升诊断准确性。
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**备注**：上述文献为领域典型研究方向示例，实际应用中建议通过**Web of Science**或**PubMed**以关键词“SERS + recombinant protein + biosensing”检索最新研究，并优先选择**Analytical Chemistry**、**Biosensors & Bioelectronics**等期刊的高被引论文。
Surface-enhanced Raman scattering (SERS)-based recombinant proteins represent an innovative integration of bioengineering and nanotechnology, offering enhanced analytical capabilities in biomedical research and diagnostics. Recombinant proteins, produced through genetic engineering in host systems like bacteria or mammalian cells, are widely used for their specificity in targeting biomolecules. SERS, on the other hand, leverages nanostructured metallic substrates to amplify weak Raman signals by orders of magnitude, enabling ultrasensitive detection of molecular interactions.
The fusion of recombinant proteins with SERS-active platforms addresses limitations in traditional detection methods, such as low sensitivity or complex labeling requirements. By conjugating recombinant proteins (e.g., antibodies, enzymes, or receptors) to SERS nanotags—typically gold or silver nanoparticles functionalized with Raman reporters—researchers create targeted probes. These probes combine the molecular recognition properties of proteins with the exceptional signal amplification of SERS, allowing precise detection of biomarkers at trace concentrations.
This synergy has revolutionized applications in early disease diagnosis, drug development, and cellular imaging. For instance, SERS-enabled recombinant antibodies can identify cancer biomarkers in serum samples with femtomolar sensitivity, outperforming conventional ELISA. Additionally, the technique supports multiplexed detection by using distinct Raman signatures for different protein targets, enabling simultaneous analysis of multiple biomarkers.
Recent advancements focus on improving biocompatibility, stability, and reproducibility of these hybrid systems. Challenges remain in standardizing protein-nanoparticle conjugation methods and minimizing non-specific binding, but ongoing research in nanomaterial engineering and protein design continues to expand their practical utility across life sciences.
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