首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAA1 |
| Uniprot No | P0DJI8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 19-122aa |
| 氨基酸序列 | RSFFSFLGEA FDGARDMWRA YSDMREANYI GSDKYFHARG NYDAAKRGPG GVWAAEAISD ARENIQRFFG HGAEDSLADQ AANEWGRSGK DPNHFRPAGL PEKY |
| 预测分子量 | 11.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SAA1重组蛋白的模拟参考文献示例(注：文献信息为虚构，仅供格式参考)：
1. **《Expression and purification of recombinant human SAA1 in Escherichia coli》**
- **作者**: Li, X., et al.
- **摘要**: 该研究通过大肠杆菌表达系统成功制备了重组人SAA1蛋白，优化了诱导条件和纯化步骤，证实其结构与天然SAA1相似，为后续功能研究提供了高纯度蛋白来源。
2. **《Pro-inflammatory role of SAA1 in macrophage activation via TLR4 signaling pathway》**
- **作者**: Wang, Y., et al.
- **摘要**: 研究发现重组SAA1可通过激活TLR4信号通路诱导巨噬细胞分泌IL-6和TNF-α，揭示了SAA1在炎症性疾病中的潜在作用机制。
3. **《Serum amyloid A1 (SAA1) as a biomarker in colorectal cancer: Insights from recombinant protein-based assays》**
- **作者**: Chen, J., et al.
- **摘要**: 利用重组SAA1蛋白开发了高灵敏度检测方法，证实其在结直肠癌患者血清中显著升高，提示其作为临床诊断标志物的潜力。
4. **《Structural characterization of recombinant SAA1 and its aggregation properties》**
- **作者**: Kim, S., et al.
- **摘要**: 通过X射线晶体学解析了重组SAA1的三维结构，并发现其在特定条件下易形成淀粉样纤维，为理解SAA1在淀粉样变性病中的病理机制提供了依据。
(注：以上文献为示例性内容，实际引用时需核实真实文献信息。)
**Background of Recombinant SAA1 Protein**
Serum Amyloid A1 (SAA1) is a highly conserved acute-phase protein predominantly synthesized by hepatocytes in response to inflammatory stimuli, such as infection, trauma, or tissue damage. As a member of the SAA family, it plays a critical role in innate immunity, acting as a chemoattractant, modulating immune cell functions, and participating in lipid metabolism. Under normal physiological conditions, SAA1 circulates at low concentrations (1–5 μg/mL), but its levels can surge up to 1.000-fold during acute inflammation.
Structurally, SAA1 is a 12–14 kDa protein composed of 104 amino acids, forming a characteristic four-helix bundle. It is closely associated with high-density lipoprotein (HDL) in plasma, influencing HDL remodeling and cholesterol transport. Dysregulation of SAA1 is implicated in chronic inflammatory diseases, amyloidosis (where it forms amyloid deposits), and cancer progression. Its overexpression has been observed in conditions like rheumatoid arthritis, atherosclerosis, and certain malignancies, making it a potential biomarker for disease monitoring.
Recombinant SAA1 protein is produced using genetic engineering techniques, often expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Purification methods typically involve affinity chromatography (e.g., His-tag systems) followed by refolding steps if necessary. Recombinant SAA1 enables researchers to study its biochemical properties, receptor interactions (e.g., TLR2/4. formyl peptide receptor 2), and signaling pathways in vitro and in vivo. It is also used to develop therapeutic strategies targeting SAA1-driven pathologies or to explore its immunomodulatory potential.
Despite its well-characterized roles, challenges remain in understanding SAA1's dual pro- and anti-inflammatory effects and its isoform-specific functions. Recombinant SAA1 tools continue to advance research into its pathophysiological mechanisms and therapeutic applications.
×
