| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPA3 |
| Uniprot No | P35244 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-119aa |
| 氨基酸序列 | MVDMMDLPRSRINAGMLAQFIDKPVCFVGRLEKIHPTGKMFILSDGEGKNGTIELMEPLDEEISGIVEVVGRVTAKATILCTSYVQFKEDSHPFDLGLYNEAVKIIHDFPQFYPLGIVQ |
| 预测分子量 | 40.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPA3重组蛋白的3篇参考文献的简单概括(注：文献为模拟示例，非真实存在)：
1. **文献名称**: "Structural and functional analysis of human RPA3 subunit in DNA repair"
**作者**: Smith J, et al.
**摘要**: 研究通过重组表达人源RPA3蛋白，解析其与RPA1/RPA2复合物的相互作用，揭示其在DNA单链损伤修复中的构象变化及功能调控机制。
2. **文献名称**: "Expression and purification of recombinant RPA3 for in vitro binding assays"
**作者**: Lee S, et al.
**摘要**: 开发了一种高效的原核表达系统用于重组RPA3蛋白的纯化，验证其与DNA及RPA复合物其他亚基的结合能力，为体外研究提供可靠工具。
3. **文献名称**: "RPA3 phosphorylation regulates replication stress response"
**作者**: Chen X, et al.
**摘要**: 利用重组RPA3蛋白突变体，发现其在DNA复制应激中的磷酸化位点对维持基因组稳定性具有关键作用，并影响与ATM/ATR信号通路的互作。
4. **文献名称**: "RPA3 deficiency disrupts telomere maintenance via impaired recombination"
**作者**: Garcia R, et al.
**摘要**: 通过重组RPA3蛋白回补实验，证明RPA3缺失导致端粒重组修复异常，揭示其在端粒保护中的特异性功能。
(注：以上文献及内容为假设性描述，实际文献需通过学术数据库检索获取。)
RPA3 (Replication Protein A3) is a critical subunit of the Replication Protein A (RPA) complex, a heterotrimeric single-stranded DNA (ssDNA)-binding protein essential for DNA metabolism. The RPA complex, composed of RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa), plays a central role in DNA replication, repair, and recombination by stabilizing ssDNA intermediates and coordinating interactions with other DNA-processing enzymes. RPA3. the smallest subunit, contributes to structural stability and functional regulation of the complex. While RPA1 and RPA2 directly mediate ssDNA binding and protein interactions, RPA3 enhances complex assembly and modulates DNA-binding affinity through conformational adjustments.
Recombinant RPA3 refers to the engineered form of this subunit produced via heterologous expression systems, such as *E. coli* or eukaryotic cell cultures. Its production enables detailed biochemical and structural studies to dissect RPA’s role in DNA damage response pathways, replication stress, and genome maintenance. Recombinant RPA3 is often co-expressed with RPA1 and RPA2 to reconstitute the functional trimeric complex for *in vitro* assays, including DNA-binding studies, replication fork restart experiments, or analyses of protein-protein interactions with repair factors like RAD52 or BRCA2.
Research on recombinant RPA3 has implications for understanding cancer biology, as RPA dysfunction is linked to genomic instability and tumorigenesis. Additionally, RPA inhibitors are being explored as potential chemotherapeutic agents. The development of recombinant RPA3 also supports diagnostic applications, such as screening for autoantibodies in autoimmune diseases where RPA components are targeted. Overall, recombinant RPA3 serves as a vital tool for unraveling DNA repair mechanisms and developing therapeutic strategies against diseases driven by DNA replication errors.
×
