| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | rbsK |
| Uniprot No | Q9H477 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-322aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAASGEPQRQWQEEVAAVVVVGSCMTDLVS LTSRLPKTGETIHGHKFFIGFGGKGANQCVQAARLGAMTSMVCKVGKDSF GNDYIENLKQNDISTEFTYQTKDAATGTASIIVNNEGQNIIVIVAGANLL LNTEDLRAAANVISRAKVMVCQLEITPATSLEALTMARRSGVKTLFNPAP AIADLDPQFYTLSDVFCCNESEAEILTGLTVGSAADAGEAALVLLKRGCQ VVIITLGAEGCVVLSQTEPEPKHIPTEKVKAVDTTGAGDSFVGALAFYLA YYPNLSLEDMLNRSNFIAAVSVQAAGTQSSYPYKKDLPLTLF |
| 预测分子量 | 36 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于rbsK重组蛋白的3篇参考文献示例(注：文献信息为模拟生成，仅供格式参考)：
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1. **文献名称**: "Cloning, expression, and purification of recombinant rbsK protein from *Escherichia coli* for kinetic characterization"
**作者**: Smith A, et al.
**摘要**: 本研究报道了通过基因克隆技术在大肠杆菌中高效表达rbsK(核糖体结合位点激酶)重组蛋白，并利用亲和层析法进行纯化。通过酶动力学实验验证了其ATP依赖性磷酸转移活性，为后续功能研究奠定基础。
2. **文献名称**: "Functional analysis of rbsK in the ribose metabolic pathway of *Bacillus subtilis*"
**作者**: Tanaka H, et al.
**摘要**: 通过重组表达枯草芽孢杆菌rbsK蛋白，探究其在D-核糖代谢中的作用。实验表明，rbsK催化核糖-5-磷酸的生成，其重组蛋白的活性受镁离子浓度显著影响，提示其在细菌碳源利用中的关键角色。
3. **文献名称**: "Optimization of recombinant rbsK production in *Pichia pastoris* for industrial applications"
**作者**: Chen L, et al.
**摘要**: 研究通过毕赤酵母系统高效表达rbsK重组蛋白，优化了发酵条件及诱导策略，使蛋白产量提升3倍。纯化后的蛋白在体外展现出稳定的热稳定性，为酶催化工艺开发提供了新方案。
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如需真实文献，建议在PubMed、Google Scholar等平台以“rbsK recombinant protein”“ribose kinase expression”等关键词检索，并筛选近5年研究以获取最新进展。
The RbsK recombinant protein is derived from the rbsK gene, which encodes ribulokinase, a key enzyme in the D-ribose utilization pathway of bacteria such as *Escherichia coli*. Ribulokinase (RbsK) catalyzes the ATP-dependent phosphorylation of D-ribulose to form D-ribulose-5-phosphate, a critical intermediate in the pentose phosphate pathway and nucleotide biosynthesis. This enzyme plays a vital role in bacterial metabolism, enabling the conversion of extracellular D-ribose into usable energy and metabolic precursors through the ribose catabolic pathway.
Recombinant RbsK is produced using genetic engineering techniques, where the rbsK gene is cloned into expression vectors (e.g., plasmid systems) and expressed in heterologous hosts like *E. coli* or yeast. The protein is typically purified via affinity chromatography, leveraging tags such as His-tags for high-yield isolation. This approach ensures scalability and consistency, making recombinant RbsK accessible for research and industrial applications.
Studies on RbsK have provided insights into bacterial sugar metabolism, enzyme kinetics, and structure-function relationships. Its recombinant form is utilized in enzymology to investigate catalytic mechanisms, substrate specificity, and allosteric regulation. Additionally, RbsK serves as a model for engineering thermostable or substrate-tolerant variants for biotechnological processes, including the production of ribose-derived compounds or synthetic biology pathways. In industrial settings, recombinant RbsK may contribute to optimizing microbial cell factories for biofuel or pharmaceutical precursor synthesis. Its structural characterization also aids in antimicrobial drug design, targeting bacterial metabolic vulnerabilities. Overall, RbsK recombinant protein exemplifies the intersection of basic science and applied biotechnology.
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