| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAF1 |
| Uniprot No | P04049 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-648aa |
| 氨基酸序列 | MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF |
| 预测分子量 | 80 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于RAF1重组蛋白的参考文献概览(基于近年研究趋势模拟，非真实文献)：
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1. **《Recombinant RAF1 Kinase Domain Expression and Structural Analysis》**
- 作者：Smith J. et al.
- 摘要：报道了通过大肠杆菌系统表达RAF1激酶结构域重组蛋白的方法，利用X射线晶体学解析其三维结构，揭示了ATP结合口袋的关键构象变化，为靶向药物设计提供依据。
2. **《Functional Characterization of RAF1 Mutants in Cancer Cell Models》**
- 作者：Li X. et al.
- 摘要：通过哺乳动物细胞表达系统制备重组RAF1突变体(如S259A)，验证其在MAPK信号通路中的异常激活机制，并证明特定突变导致肿瘤细胞对MEK抑制剂的耐药性。
3. **《Purification and Activity Assay of RAF1 Recombinant Protein for Drug Screening》**
- 作者：Garcia R. et al.
- 摘要：开发了基于昆虫细胞-杆状病毒系统的RAF1重组蛋白高纯度制备流程，建立体外激酶活性检测平台，用于高通量筛选RAF1小分子抑制剂。
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注：以上内容为模拟示例，实际文献需通过PubMed或Web of Science等平台检索确认。
RAF1 (also known as CRAF or c-Raf) is a serine/threonine-protein kinase that plays a pivotal role in the RAS-RAF-MEK-ERK signaling cascade, a critical pathway regulating cell proliferation, differentiation, survival, and apoptosis. As a member of the RAF kinase family, RAF1 is activated by RAS GTPases in response to extracellular stimuli, such as growth factors or cytokines. Upon activation, it phosphorylates and activates MEK1/2. which subsequently activates ERK1/2. leading to downstream transcriptional and metabolic responses. Dysregulation of this pathway, often due to mutations in RAF1 or related components, is implicated in numerous cancers and developmental disorders.
Recombinant RAF1 protein is engineered through molecular cloning and expression in heterologous systems (e.g., E. coli, insect, or mammalian cells) to produce functional, purified kinase for research and therapeutic applications. Its recombinant form retains key structural domains: three conserved regions (CR1-CR3), including a RAS-binding domain (CR1) and a kinase domain (CR3), essential for its activation and signaling functions. Researchers utilize recombinant RAF1 to study kinase activity, pathway interactions, and mutations (e.g., oncogenic variants like RAF1 S259L) linked to Noonan syndrome or cancers. It also serves as a tool for high-throughput drug screening to identify RAF inhibitors, with implications for targeting RAF-driven malignancies. Additionally, structural studies of recombinant RAF1 have advanced understanding of its regulation, including autoinhibition mechanisms and dimerization processes critical for pathway activation. Its role in resistance to BRAF inhibitors (e.g., in melanoma) further underscores its therapeutic relevance. Overall, recombinant RAF1 is a vital resource for dissecting RAF biology and developing precision oncology therapies.
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