| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAE1 |
| Uniprot No | P78406 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-368aa |
| 氨基酸序列 | MSLFGTTSGFGTSGTSMFGSATTDNHNPMKDIEVTSSPDDSIGCLSFSPPTLPGNFLIAGSWANDVRCWEVQDSGQTIPKAQQMHTGPVLDVCWSDDGSKVFTASCDKTAKMWDLSSNQAIQIAQHDAPVKTIHWIKAPNYSCVMTGSWDKTLKFWDTRSSNPMMVLQLPERCYCADVIYPMAVVATAERGLIVYQLENQPSEFRRIESPLKHQHRCVAIFKDKQNKPTGFALGSIEGRVAIHYINPPNPAKDNFTFKCHRSNGTNTSAPQDIYAVNGIAFHPVHGTLATVGSDGRFSFWDKDARTKLKTSEQLDQPISACCFNHNGNIFAYASSYDWSKGHEFYNPQKKNYIFLRNAAEELKPRNKK |
| 预测分子量 | 68.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAE1重组蛋白的3篇参考文献的简要概括：
1. **文献名称**: "RAE1 promotes HBV transcription and replication by enhancing the assembly of viral capsid"
**作者**: Zhang Y, et al.
**摘要**: 该研究利用重组RAE1蛋白，揭示了其在乙型肝炎病毒(HBV)复制中的作用机制，发现RAE1通过与病毒核心蛋白相互作用，促进病毒衣壳组装及RNA转录。
2. **文献名称**: "RAE1 interacts with NUMA1 to regulate bipolar spindle formation and chromosome segregation"
**作者**: Wong RW, et al.
**摘要**: 研究者通过重组RAE1蛋白实验，证明其与核基质蛋白NUMA1的结合对细胞有丝分裂中纺锤体形成和染色体分离具有关键调控作用，为癌症治疗提供潜在靶点。
3. **文献名称**: "The mRNA export factor RAE1 contributes to aggressive tumor phenotypes and poor prognosis in colorectal cancer"
**作者**: Wang L, et al.
**摘要**: 该研究通过重组RAE1蛋白功能实验，发现其过表达通过激活Wnt/β-catenin通路促进结直肠癌细胞迁移和侵袭，与患者预后不良显著相关。
注：以上文献为示例，实际引用时需核对具体来源及发表年份。若需准确文献，建议通过PubMed或Web of Science以“RAE1 recombinant protein”为关键词检索。
**Background of RAE1 Recombinant Protein**
RAE1 (RNA export adapter protein 1), also known as RAE1 or hnRNP-associated nuclear pore complex protein, is a conserved multifunctional protein involved in mRNA export, cell cycle regulation, and maintenance of genomic stability. Structurally, RAE1 contains WD40 repeat domains that mediate interactions with nucleoporins (e.g., Nup98) and other partners, positioning it as a critical component of the nuclear pore complex (NPC). It facilitates mRNA transport from the nucleus to the cytoplasm by bridging mRNA molecules and NPC components.
Beyond mRNA export, RAE1 plays a role in mitotic regulation. During mitosis, RAE1 interacts with the Sec13 subunit of the Nup107-160 nuclear pore subcomplex and participates in spindle assembly and kinetochore function. It also associates with the anaphase-promoting complex/cyclosome (APC/C), a key E3 ubiquitin ligase, to regulate mitotic progression. Dysregulation of RAE1 disrupts the spindle assembly checkpoint, leading to chromosomal instability—a hallmark of cancer.
RAE1 is implicated in viral pathogenesis, as certain viruses (e.g., herpesviruses) exploit its mRNA export function to promote viral RNA trafficking. In cancer, RAE1 overexpression correlates with poor prognosis, tumor progression, and metastasis, likely due to its dual roles in mRNA processing and mitotic fidelity.
Recombinant RAE1 protein is engineered for research applications, enabling studies on nuclear transport mechanisms, cell cycle control, and viral-host interactions. Its recombinant form is pivotal in structural studies, drug screening, and functional assays to explore therapeutic targeting of RAE1 in cancer or antiviral strategies. Ongoing research continues to unravel its regulatory networks and potential as a biomarker or therapeutic target.
×
