| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAD23A |
| Uniprot No | P54725 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-362aa |
| 氨基酸序列 | MAVTITLKTLQQQTFKIRMEPDETVKVLKEKIEAEKGRDAFPVAGQKLIYAGKILSDDVPIRDYRIDEKNFVVVMVTKTKAGQGTSAPPEASPTAAPESSTSFPPAPTSGMSHPPPAAREDKSPSEESAPATSPESVSGSVPSSGSSGREEDAASTLVTGSEYETMLTEIMSMGYERERVVAALRASYNNPHRAVEYLLTGIPGSPEPEHGSVQESQVSEQPATEAGENPLEFLRDQPQFQNMRQVIQQNPALLPALLQQLGQENPQLLQQISRHQEQFIQMLNEPPGELADISDVEGEVGAIGEEAPQMNYIQVTPQEKEAIERLKALGFPESLVIQAYFACEKNENLAANFLLSQNFDDE |
| 预测分子量 | 47.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAD23A重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*RAD23A promotes nucleotide excision repair by coordinating assembly of the repair complex*
**作者**：Sugasawa, K. et al.
**摘要**：该研究阐明了RAD23A重组蛋白在核苷酸切除修复(NER)中的作用，发现其通过结合XPC蛋白形成复合体，增强DNA损伤识别能力，并促进后续修复因子的招募。
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2. **文献名称**：*Structural and functional analysis of the RAD23-XPC complex in DNA damage recognition*
**作者**：Hey, T. et al.
**摘要**：通过表达纯化RAD23A重组蛋白，解析其与XPC形成的复合体结构，揭示了RAD23A通过泛素结合结构域(UBL)调控XPC稳定性，从而优化损伤DNA的结合效率。
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3. **文献名称**：*RAD23A links the ubiquitin-proteasome system to stress granule dynamics*
**作者**：Chen, L. et al.
**摘要**：研究利用重组RAD23A蛋白验证其与泛素化底物及蛋白酶体的相互作用，提出RAD23A通过调控错误折叠蛋白的降解，参与细胞应激颗粒的形成与分解过程。
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以上文献均聚焦RAD23A重组蛋白的功能机制，涵盖DNA修复、结构互作及蛋白酶体调控等方向。
**Background of RAD23A Recombinant Protein**
RAD23A (RAD23 homolog A) is a critical protein involved in DNA damage recognition and repair, primarily functioning within the nucleotide excision repair (NER) pathway. It belongs to the RAD23 family, which includes two human paralogs, RAD23A and RAD23B. These proteins act as shuttle factors, bridging damaged DNA and the proteasome-mediated degradation machinery. RAD23A contains two conserved domains: an N-terminal ubiquitin-associated (UBA) domain and a ubiquitin-like (UBL) domain, which facilitate interactions with ubiquitinated substrates and the 26S proteasome, respectively.
A key role of RAD23A is its partnership with XPC (xeroderma pigmentosum group C protein) in NER. RAD23A stabilizes XPC, enhancing its ability to recognize and bind to DNA lesions caused by UV-induced damage or chemical adducts. This interaction is essential for initiating the repair of bulky DNA lesions, preventing mutagenesis and maintaining genomic stability. Beyond DNA repair, RAD23A is implicated in regulating protein turnover via the ubiquitin-proteasome system, linking protein quality control to genomic integrity.
Dysregulation of RAD23A has been associated with cancer progression and neurodegenerative diseases. For instance, reduced RAD23A expression may impair DNA repair, increasing cancer risk, while its overexpression has been observed in certain tumors. Recombinant RAD23A protein, produced through heterologous expression systems (e.g., *E. coli* or mammalian cells), is widely used in *in vitro* studies to dissect its molecular interactions, structural features, and functional mechanisms. It also serves as a tool for screening therapeutic agents targeting DNA repair pathways or proteasome-related disorders.
Research on RAD23A continues to uncover its multifaceted roles in cellular homeostasis, making it a protein of significant interest in cancer biology, aging, and molecular therapeutics.
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