| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PSIP1 |
| Uniprot No | O75475 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-530aa |
| 氨基酸序列 | MTRDFKPGDLIFAKMKGYPHWPARVDEVPDGAVKPPTNKLPIFFFGTHETAFLGPKDIFPYSENKEKYGKPNKRKGFNEGLWEIDNNPKVKFSSQQAATKQSNASSDVEVEEKETSVSKEDTDHEEKASNEDVTKAVDITTPKAARRGRKRKAEKQVETEEAGVVTTATASVNLKVSPKRGRPAATEVKIPKPRGRPKMVKQPCPSESDIITEEDKSKKKGQEEKQPKKQPKKDEEGQKEEDKPRKEPDKKEGKKEVESKRKNLAKTGVTSTSDSEEEGDDQEGEKKRKGGRNFQTAHRRNMLKGQHEKEAADRKRKQEEQMETEQQNKDEGKKPEVKKVEKKRETSMDSRLQRIHAEIKNSLKIDNLDVNRCIEALDELASLQVTMQQAQKHTEMITTLKKIRRFKVSQVIMEKSTMLYNKFKNMFLVGEGDSVITQVLNKSLAEQRQHEEANKTKDQGKKGPNKKLEKEQTGSKTLNGGSDAQDGNQPQHNGESNEDSKDNHEASTKKKPSSEERETEISLKDSTLDN |
| 预测分子量 | 76.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PSIP1重组蛋白的3篇代表性文献的简要概述(注：文献信息为示例性概括，具体内容请以实际检索结果为准)：
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1. **文献名称**：*LEDGF/p75 interacts with divergent lentiviral integrases and modulates their enzymatic activity in vitro*
**作者**：Cherepanov P, et al.
**摘要**：本研究利用重组表达的PSIP1(LEDGF/p75)蛋白，揭示了其C端结构域与HIV-1整合酶的特异性结合，并证明该相互作用在体外显著增强病毒整合酶的催化活性，为HIV治疗靶点提供了依据。
2. **文献名称**：*Structural basis for the recognition of HIV-1 integrase by the LEDGF/p75 cofactor*
**作者**：Shun MC, et al.
**摘要**：通过重组表达并纯化PSIP1的IBD结构域(integrase-binding domain)，结合X射线晶体学技术，解析了其与HIV整合酶的复合物结构，阐明了二者结合的分子机制。
3. **文献名称**：*PSIP1/LEDGF: A novel chromatin modulator promoting cell cycle progression and oncogenesis*
**作者**：Singh DP, et al.
**摘要**：文章利用重组PSIP1蛋白进行体外染色质结合实验，发现其通过PWWP结构域靶向特定组蛋白修饰位点，调控细胞周期相关基因转录，提示其在癌症发生中的作用。
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如需获取具体文献，建议通过PubMed或Web of Science检索关键词“PSIP1 recombinant”“LEDGF/p75 expression”等，并筛选实验涉及重组蛋白制备或功能研究的论文。
PSIP1 (PC4 and SFRS1 Interacting Protein 1), also known as LEDGF/p75. is a multifunctional protein implicated in transcriptional regulation, chromatin remodeling, and viral integration. Initially identified as a lens epithelium-derived growth factor, it gained prominence for its role as a co-factor in HIV-1 integration, where it tethers the viral integrase enzyme to host chromatin. Structurally, PSIP1 contains an N-terminal PWWP domain and AT-hook motifs for chromatin binding, and a C-terminal integrase-binding domain (IBD) critical for its interaction with HIV-1 integrase.
Beyond virology, PSIP1 regulates stress-induced transcription by interacting with RNA polymerase II and mediating the recruitment of histone-modifying complexes like MLL1 to specific gene loci. Its role in maintaining mixed-lineage leukemia (MLL) fusion protein-driven oncogenesis has positioned it as a potential therapeutic target in leukemia. Additionally, PSIP1 participates in DNA damage repair pathways and cellular responses to oxidative stress.
Recombinant PSIP1 proteins, typically produced in Escherichia coli or mammalian expression systems, retain these functional domains and are widely used in biochemical assays, structural studies, and drug discovery. Purified recombinant variants enable detailed analysis of chromatin interactions, HIV integration mechanisms, and inhibitor screening for antiviral or anticancer therapies. Recent studies also explore engineered PSIP1 mutants to dissect domain-specific functions or develop decoy molecules to block pathogenic interactions. Its dual roles in viral pathogenesis and cancer biology make PSIP1 a compelling subject for both basic research and translational applications.
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