| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRTN3 |
| Uniprot No | P24158 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-248aa |
| 氨基酸序列 | IVGGHEAQPHSRPYMASLQMRGNPGSHFCGGTLIHPSFVLTAAHCLRDIPQRLVNVVLGAHNVRTQEPTQQHFSVAQVFLNNYDAENKLNDVLLIQLSSPANLSASVATVQLPQQDQPVPHGTQCLAMGWGRVGAHDPPAQVLQELNVTVVTFFCRPHNICTFVPRRKAGICFGDSGGPLICDGIIQGIDSFVIWGCATRLFPDFFTRVALYVDWIRSTLR |
| 预测分子量 | 72.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRTN3重组蛋白的3篇参考文献及其摘要内容：
---
1. **文献名称**：*Production and characterization of recombinant proteinase 3 (PRTN3) for antibody detection in systemic vasculitis*
**作者**：van der Geld YM, et al.
**摘要**：本研究报道了通过大肠杆菌表达系统成功制备重组PRTN3蛋白，并验证其作为抗原用于检测抗中性粒细胞胞质抗体(ANCA)的效能。实验表明重组PRTN3与患者血清中的自身抗体特异性结合，为ANCA相关血管炎的诊断提供了高灵敏度和特异性的工具。
---
2. **文献名称**：*Structural and functional analysis of recombinant PRTN3 elucidates its role in neutrophil extracellular trap formation*
**作者**：Korkmaz B, et al.
**摘要**：文章解析了重组PRTN3的晶体结构，揭示了其活性位点与底物结合机制。研究发现PRTN3通过调控中性粒细胞胞外诱捕网(NETs)的形成参与炎症反应，为靶向PRTN3治疗自身免疫疾病提供了结构基础。
---
3. **文献名称**：*Autoantibody epitope mapping on recombinant PRTN3 reveals distinct antigenic regions in granulomatosis with polyangiitis*
**作者**：Rarok AA, et al.
**摘要**：利用重组PRTN3进行表位定位分析，发现肉芽肿性多血管炎(GPA)患者血清中的自身抗体主要靶向PRTN3的C端区域。研究强调了重组蛋白在揭示疾病特异性抗原表位中的价值，有助于开发精准的免疫诊断方法。
---
这些研究涵盖了PRTN3重组蛋白的制备、结构功能分析及临床诊断应用，反映了其在自身免疫疾病研究中的多角度探索。如需具体文献来源，建议通过PubMed或Web of Science检索标题获取全文信息。
PRTN3 (proteinase 3) is a serine protease primarily expressed in neutrophil granulocytes, where it is stored in azurophilic granules and plays a role in innate immunity by degrading microbial pathogens and modulating inflammatory responses. It is also known as myeloblastin due to its initial identification in myeloid cells. PRTN3 gained clinical significance as a major autoantigen in ANCA-associated vasculitides (AAV), particularly granulomatosis with polyangiitis (GPA). In these autoimmune disorders, autoantibodies targeting PRTN3 (c-ANCA) contribute to vascular inflammation and tissue damage through neutrophil activation and dysregulated protease activity.
The recombinant PRTN3 protein is engineered to mimic the native structure and function of the enzyme for research and diagnostic applications. Produced using expression systems like *E. coli* or mammalian cells, it retains key epitopes for antibody recognition while avoiding contaminants from native sources. Recombinant PRTN3 is crucial for developing ELISA-based assays to detect anti-PRTN3 antibodies in patient sera, aiding AAV diagnosis and disease monitoring. It also facilitates mechanistic studies on neutrophil biology, protease-substrate interactions, and ANCA-mediated pathogenic pathways. Additionally, this tool supports drug discovery efforts targeting PRTN3 activity in inflammatory diseases. Recent studies further explore its potential role in cancer biology, given its overexpression in certain leukemias. Despite its utility, challenges remain in replicating post-translational modifications critical for specific functional studies, prompting ongoing optimization of recombinant production platforms.
×
