| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP3CC |
| Uniprot No | P48454 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-512aa |
| 氨基酸序列 | SGRRFHLSTTDRVIKAVPFPPTQRLTFKEVFENGKPKVDVLKNHLVKEGRLEEEVALKIINDGAAILRQEKTMIEVDAPITVCGDIHGQFFDLMKLFEVGGSPSNTRYLFLGDYVDRGYFSIECVLYLWSLKINHPKTLFLLRGNHECRHLTDYFTFKQECRIKYSEQVYDACMETFDCLPLAALLNQQFLCVHGGMSPEITSLDDIRKLDRFTEPPAFGPVCDLLWSDPSEDYGNEKTLEHYTHNTVRGCSYFYSYPAVCEFLQNNNLLSIIRAHEAQDAGYRMYRKSQATGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELISDDEAEGSTTVRKEIIRNKIRAIGKMARVFSILRQESESVLTLKGLTPTGTLPLGVLSGGKQTIETATVEAVEAREAIRGFSLQHKIRSFEEARGLDRINERMPPRKDSIHAGGPMKSVTSAHSHAAHRSDQGKKAHS |
| 预测分子量 | 62.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP3CC重组蛋白的模拟参考文献示例(基于研究领域常见方向，非真实文献)：
1. **"Recombinant PPP3CC Expression and Its Role in Calcineurin Signaling"**
- 作者：Lee, J. et al.
- 摘要：研究通过大肠杆菌系统成功表达并纯化重组PPP3CC蛋白，验证其在钙离子/钙调素依赖的磷酸酶活性，揭示其在T细胞活化信号通路中的调控作用。
2. **"Structural and Functional Analysis of PPP3CC in Neuronal Development"**
- 作者：Martinez, R. & Zhang, Y.
- 摘要：利用重组PPP3CC蛋白进行体外酶动力学实验，发现其与突触蛋白相互作用，调节神经元树突发育，并解析了关键催化结构域的功能。
3. **"PPP3CC Genetic Variants and Recombinant Protein Dysfunction in Schizophrenia"**
- 作者：Cohen, S. et al.
- 摘要：通过构建携带精神分裂症相关突变的PPP3CC重组蛋白，发现其磷酸酶活性显著降低，提示基因变异可能导致神经信号异常。
4. **"High-Throughput Screening of PPP3CC Inhibitors Using Recombinant Protein Assays"**
- 作者：Wang, H. et al.
- 摘要：开发基于重组PPP3CC的荧光底物检测体系，筛选出小分子抑制剂，为免疫抑制或神经疾病药物研发提供候选分子。
**注意**：以上为模拟内容，实际文献需通过PubMed、Google Scholar等平台检索关键词“PPP3CC recombinant protein”或“PPP3CC calcineurin”获取。
**Background of PPP3CC Recombinant Protein**
PPP3CC (Protein Phosphatase 3 Catalytic Subunit C) encodes the gamma isoform of the catalytic subunit of calcineurin, a calcium/calmodulin-dependent serine/threonine phosphatase. Calcineurin plays critical roles in cellular signaling, particularly in calcium-mediated pathways, influencing processes such as T-cell activation, neuronal development, and cardiac hypertrophy. The PPP3CC subunit forms a heterodimer with regulatory subunit B (calcineurin B), requiring calcium and calmodulin for activation.
Recombinant PPP3CC protein is engineered to study calcineurin's structure, function, and interactions. Its production typically involves heterologous expression systems (e.g., *E. coli* or mammalian cells*) to generate high-purity, bioactive protein for *in vitro* assays. Researchers utilize PPP3CC recombinant protein to investigate enzymatic kinetics, screen inhibitors (e.g., cyclosporine A, FK506 analogs), and explore its role in diseases.
PPP3CC is linked to neuropsychiatric disorders (e.g., schizophrenia, bipolar disorder) and immune dysregulation. Genetic variants may alter calcineurin signaling, affecting synaptic plasticity and neurotransmitter release. In cancer, PPP3CC overexpression or suppression correlates with tumor progression, making it a potential therapeutic target.
Overall, PPP3CC recombinant protein serves as a vital tool for dissecting calcineurin’s multifunctional biology and its implications in health and disease.
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