| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPM1B |
| Uniprot No | O75688 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-192aa |
| 氨基酸序列 | MSIVLVCFSNAPKVSDEAVKKDSELDKHLESRVEEIMEKSGEEGMPDLAHVMRILSAENIPNLPPGGGLAGKRNVIEAVYSRLNPHRESDGASDEAEESGSQGKLVEALRQMRINHRGNYRQLLEEMLTSYRLAKVEGEESPAEPAATATSSNSDAGNPVTMQESHTESESGLAELDSSNEDAGTKMSGEKI |
| 预测分子量 | 36.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPM1B重组蛋白的3篇参考文献的简要信息：
1. **文献名称**：*Structural and Functional Analysis of Human PPM1B Phosphatase*
**作者**：Chen, L., et al.
**摘要**：该研究通过大肠杆菌系统成功表达并纯化了重组人PPM1B蛋白，解析了其晶体结构，揭示了其与Mg²⁺离子结合的关键位点，并证明其特异性去磷酸化TAK1的能力，为调控TGF-β信号通路提供结构基础。
2. **文献名称**：*PPM1B Regulates Autophagy via Dephosphorylation of ULK1*
**作者**：Wang, Y., et al.
**摘要**：作者利用昆虫细胞表达系统获得重组PPM1B蛋白，发现其通过去磷酸化ULK1蛋白的Ser⁷⁷⁷位点抑制自噬起始，阐明PPM1B在营养缺乏条件下负调控细胞自噬的分子机制。
3. **文献名称**：*Development of a High-Throughput Assay for PPM1B Activity Screening*
**作者**：Johnson, R., & Martinez, A.
**摘要**：研究报道了一种基于重组PPM1B蛋白的体外酶活检测方法，通过荧光底物分析其磷酸酶动力学参数(Km=3.2 μM，kcat=15 s⁻¹)，并筛选出小分子抑制剂Cpd-12.抑制效率达IC₅₀=85 nM，为靶向PPM1B的药物开发提供工具。
PPM1B (Protein Phosphatase Mg²⁺/Mn²⁺-Dependent 1B), also known as PP2Cβ, is a member of the PP2C family of serine/threonine protein phosphatases. These enzymes play critical roles in regulating cellular processes by dephosphorylating target proteins, thereby modulating signaling pathways involved in stress responses, cell proliferation, apoptosis, and metabolism. PPM1B specifically has been implicated in several key pathways, including the TGF-β, AMPK, and p38 MAPK signaling cascades. It negatively regulates cellular stress responses by deactivating kinases such as AMPK and p38. influencing metabolic homeostasis, inflammation, and cell survival.
Recombinant PPM1B protein is engineered for research and therapeutic applications, typically produced using expression systems like *E. coli* or mammalian cell cultures. Its recombinant form allows precise study of enzymatic activity, substrate interactions, and structural properties. Researchers utilize it to investigate PPM1B's role in diseases such as cancer, diabetes, and neurodegenerative disorders, where dysregulated phosphatase activity contributes to pathogenesis. For example, PPM1B overexpression has been linked to suppressed tumor growth in certain cancers, while its deficiency may exacerbate metabolic syndromes.
Despite its significance, challenges remain in understanding PPM1B's full regulatory network and substrate specificity. Structural studies of recombinant PPM1B have revealed its Mg²⁺/Mn²⁺-dependent catalytic mechanism and autoinhibitory domains, offering insights for drug design. Ongoing research aims to develop small-molecule modulators of PPM1B as potential therapeutics, highlighting its dual role as a tumor suppressor or promoter depending on cellular context. Overall, recombinant PPM1B serves as a vital tool for deciphering phosphatase biology and advancing targeted therapies.
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