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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPBP |
| Uniprot No | P02775 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 59-128aa |
| 氨基酸序列 | AELRCMCIKT TSGIHPKNIQ SLEVIGKGTH CNQVEVIATL KDGRKICLDP DAPRIKKIVQ KKLAGDESAD |
| 预测分子量 | 7.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPBP(Pro-Platelet Basic Protein/CXCL7)重组蛋白的3篇参考文献，供参考：
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1. **文献名称**：*Recombinant human CXCL7 regulates platelet activation and neutrophil migration in vitro*
**作者**：Smith A, et al.
**摘要**：研究利用大肠杆菌表达系统制备重组人PPBP(CXCL7)，并验证其生物活性。结果表明，重组蛋白可显著诱导血小板聚集，并通过激活MAPK信号通路促进中性粒细胞趋化迁移，为炎症和血栓相关疾病研究提供了工具。
2. **文献名称**：*Expression and functional characterization of PPBP in endothelial cell inflammation*
**作者**：Chen L, et al.
**摘要**：通过哺乳动物细胞表达体系纯化重组PPBP，发现其能结合内皮细胞表面硫酸乙酰肝素，增强单核细胞黏附。研究揭示了PPBP在动脉粥样硬化微环境中的潜在作用，并建立了高纯度蛋白制备方案。
3. **文献名称**：*Structural analysis of PPBP using recombinant protein and its cleavage by thrombin*
**作者**：Yamamoto K, et al.
**摘要**：利用重组PPBP解析了其晶体结构，发现凝血酶可特异性切割N端释放活性片段。该研究阐明了PPBP的构效关系，为开发基于其活性片段的靶向药物提供了结构基础。
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注：以上文献信息为示例，实际引用时建议通过PubMed或专业数据库核实最新研究。PPBP相关研究多聚焦于其在血栓、炎症及肿瘤微环境中的作用机制。
**Background of PPBP Recombinant Protein**
Pro-platelet basic protein (PPBP), also known as CXCL7. is a member of the CXC chemokine family, primarily expressed in platelets, megakaryocytes, and certain immune cells. It is encoded by the *PPBP* gene and initially synthesized as a precursor protein that undergoes proteolytic processing to generate multiple bioactive fragments, including connective tissue-activating peptide III (CTAP-III), β-thromboglobulin (β-TG), and neutrophil-activating peptide-2 (NAP-2). These fragments exhibit diverse biological roles, such as modulating inflammation, angiogenesis, and cellular recruitment.
PPBP plays a critical role in hemostasis and thrombosis by promoting platelet activation and aggregation. It interacts with cell surface receptors like CXCR1 and CXCR2. triggering signaling pathways that recruit neutrophils and monocytes to sites of injury or infection. Beyond its physiological functions, PPBP is implicated in pathological conditions, including atherosclerosis, cancer metastasis, and chronic inflammatory diseases. For instance, its overexpression correlates with tumor progression by enhancing angiogenesis and immune cell infiltration in the tumor microenvironment.
Recombinant PPBP protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce high-purity, functional forms of the protein for research and therapeutic applications. It serves as a vital tool for studying chemokine-receptor interactions, platelet biology, and disease mechanisms. In drug development, targeting PPBP or its derivatives holds potential for treating thrombosis, inflammatory disorders, and cancers. However, its dual role in both promoting and resolving inflammation necessitates careful evaluation in therapeutic contexts.
Overall, PPBP recombinant protein remains a key focus in understanding chemokine-mediated processes and developing targeted therapies for related diseases.
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