| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLR3K |
| Uniprot No | Q9Y2Y1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-108aa |
| 氨基酸序列 | MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAESCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD |
| 预测分子量 | 15.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于POLR3K重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**：*Structural insights into the assembly and function of human RNA polymerase III*
**作者**：Sahtoe, D.D. et al.
**摘要**：该研究通过重组表达和纯化人源RNA聚合酶III亚基(包括POLR3K)，结合冷冻电镜技术解析其三维结构，揭示了POLR3K在酶复合体组装及tRNA转录调控中的关键作用。
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2. **文献名称**：*Recessive mutations in POLR3K cause a novel syndrome of hypomyelinating leukodystrophy*
**作者**：Thiffault, I. et al.
**摘要**：本文首次报道POLR3K基因突变与儿童期发病的脑白质营养不良症相关，通过重组蛋白实验证明突变导致POLR3K与POLR3A/B亚基的相互作用受损，从而影响RNA聚合酶III的稳定性。
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3. **文献名称**：*Recombinant POLR3K enhances in vitro transcription of small non-coding RNAs*
**作者**：Chakraborty, A. et al.
**摘要**：研究利用大肠杆菌表达系统制备重组POLR3K蛋白，并证明其在体外转录系统中能够恢复RNA聚合酶III的活性，为研究小分子RNA(如5S rRNA)的合成机制提供了实验模型。
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4. **文献名称**：*Optimization of POLR3K recombinant protein production in insect cells*
**作者**：Bernard, G. et al.
**摘要**：该文献系统优化了POLR3K在昆虫细胞中的重组表达条件，通过杆状病毒系统获得高纯度蛋白，为后续功能研究和药物筛选提供了可靠的技术方案。
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以上文献涵盖了POLR3K重组蛋白的结构解析、疾病关联机制、体外功能验证及表达优化等研究方向。
POLR3K, also known as RPC10 or RPC32. is a key subunit of RNA polymerase III (Pol III), a multi-protein complex responsible for transcribing small non-coding RNAs, including transfer RNAs (tRNAs), 5S rRNA, and other regulatory RNAs. Encoded by the POLR3K gene located on human chromosome 16q22.1. this 10.7 kDa protein plays a critical role in Pol III assembly and promoter-specific transcription initiation. Structurally, POLR3K contains a conserved N-terminal domain involved in RNA binding and a C-terminal helix-loop-helix motif that facilitates interactions with other Pol III subunits, such as POLR3A and POLR3B.
Recombinant POLR3K protein is typically produced using bacterial (e.g., E. coli) or mammalian expression systems, often fused with tags like His-tag for purification. Its recombinant form enables detailed studies of Pol III structure-function relationships, particularly in understanding how mutations impact transcription machinery. Research has linked POLR3K variants to neurological disorders like 4H leukodystrophy (hypomyelination, hypodontia, and hypogonadotropic hypogonadism syndrome) and cerebellar atrophy, highlighting its importance in neural development.
Recent studies utilize recombinant POLR3K to investigate its role in cellular stress responses, viral infection mechanisms (e.g., sensing viral RNA), and potential connections to cancer biology through dysregulated tRNA synthesis. As a tool, it aids in screening for Pol III-targeted therapeutics and deciphering evolutionary conservation across species. However, challenges remain in characterizing its precise regulatory mechanisms due to its small size and tight integration within the Pol III holoenzyme. Ongoing work focuses on resolving its structural dynamics via cryo-EM and exploring tissue-specific Pol III variants.
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