| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | Pdia2 |
| Uniprot No | D3Z6P0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-527aa |
| 氨基酸序列 | QGEEPGGPSEVLPEEPTGEEVPKEDGILVLNHRTLSLALQEHSALMVEFYAPWCGHCKELAPEYSKAAALLAAESAVVTLAKVDGPAEPELTKEFEVVGYPTLKFFQNGNRTNPEEYAGPKTAEGIAEWLRRRVGPSATHLEDEEGVQALMAKWDMVVIGFFQDLQGKDMATFLALAKDALDMTFGFTDQPQLFEKFGLTKDTVVLFKKFDEGRADFPVDKETGLDLGDLSRFLVIHSMHLVTEFNSQTSPKIFAAKILNHLLLFVNQTLAQHRELLTDFREAAPPFRGQVLFVMVDVAADNSHVLNYFGLKAEEAPTLRLINVETTKKYAPTGVIAITAASVAAFCQAVLHGEIKHYLLSQEIPPDWDQGPVKTLVSKNFEQVAFDETKNVFVKFYAPWCSHCKEMAPAWEALAEKYKDREDIVIAELDATANELEAFSVLGYPTLKFFPAGPDRKVIDYKSTRDLETFSKFLDSGGHLPKEEPKEPAASAPEAQANSTLGPKEEL |
| 预测分子量 | 59.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PDIA2重组蛋白的3篇参考文献的简要概括：
1. **文献名称**：*Recombinant PDIA2 expression and characterization in oxidative protein folding*
**作者**：Smith J, et al.
**摘要**：研究利用大肠杆菌系统表达重组PDIA2蛋白，验证其作为二硫键异构酶的活性。实验表明，PDIA2在体外能够催化胰岛素还原变性后的复性，并通过硫氧还蛋白结构域的突变分析揭示了其底物结合的关键区域。
2. **文献名称**：*PDIA2 modulates ER stress in cancer cells via interaction with HER2*
**作者**：Li X, et al.
**摘要**：该文献发现重组PDIA2蛋白与HER2受体在乳腺癌细胞中相互作用，通过调节内质网应激通路影响肿瘤细胞存活。研究利用免疫共沉淀和基因敲除技术，证实PDIA2可能成为癌症治疗的潜在靶点。
3. **文献名称**：*Structural insights into PDIA2 substrate specificity by cryo-EM*
**作者**：Wang Y, et al.
**摘要**：通过冷冻电镜解析重组PDIA2蛋白的三维结构，揭示其底物结合口袋的独特构象。研究发现PDIA2对富含半胱氨酸的底物(如免疫球蛋白)具有高亲和力，为设计特异性抑制剂提供结构基础。
注：以上文献信息为模拟示例，实际文献需通过数据库(如PubMed、Web of Science)检索确认。若需具体文献，建议使用关键词“PDIA2 recombinant protein”或“PDIA2 structural/functional study”进一步查询。
**Background of PDIA2 Recombinant Protein**
Protein Disulfide Isomerase A2 (PDIA2), a member of the protein disulfide isomerase (PDI) family, plays a critical role in catalyzing disulfide bond formation, isomerization, and rearrangement during protein folding in the endoplasmic reticulum (ER). PDIA2 is particularly noted for its expression in reproductive tissues, such as the testes and ovaries, as well as in secretory cells, where it supports the maturation of proteins requiring structural disulfide bonds. Unlike other PDI family members (e.g., PDIA1), PDIA2 exhibits tissue-specific expression patterns, suggesting specialized roles in cellular processes unique to these tissues.
Structurally, PDIA2 contains thioredoxin-like domains with catalytic CXXC motifs, enabling redox activity essential for its chaperone and isomerase functions. Its recombinant form is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure proper folding and post-translational modifications. Recombinant PDIA2 retains enzymatic activity, making it a valuable tool for studying ER stress, protein misfolding disorders (e.g., neurodegenerative diseases), and reproductive biology.
Research applications include *in vitro* assays to investigate its interaction with client proteins, redox signaling pathways, and potential therapeutic targeting in diseases linked to ER dysfunction. Additionally, PDIA2's role in sperm maturation and fertilization has spurred interest in reproductive medicine. The availability of recombinant PDIA2 facilitates mechanistic studies, drug screening, and the development of biologics aimed at modulating disulfide bond-dependent processes in health and disease.
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