| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OTX2 |
| Uniprot No | P32243 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-297aa |
| 氨基酸序列 | MMSYLKQPPYAVNGLSLTTSGMDLLHPSVGYPGPWASCPAATPRKQRRERTTFTRAQLDVLEALFAKTRYPDIFMREEVALKINLPESRVQVWFKNRRAKCRQQQQQQQNGGQNKVRPAKKKTSPAREVSSESGTSGQFTPPSSTSVPTIASSSAPVSIWSPASISPLSDPLSTSSSCMQRSYPMTYTQASGYSQGYAGSTSYFGGMDCGSYLTPMHHQLPGPGATLSPMGTNAVTSHLNQSPASLSTQGYGASSLGFNSTTDCLDYKDQTASWKLNFNADCLDYKDQTSSWKFQVL |
| 预测分子量 | 48.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OTX2重组蛋白的模拟参考文献示例(实际文献需通过学术数据库查询)：
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1. **文献名称**: *"Expression and functional characterization of recombinant human OTX2 transcription factor in Escherichia coli"*
**作者**: Smith A, et al.
**摘要**: 本研究利用大肠杆菌系统成功表达并纯化了OTX2重组蛋白，通过电泳迁移实验(EMSA)证实其与靶DNA序列的特异性结合能力，为后续功能研究提供工具。
2. **文献名称**: *"OTX2 recombinant protein regulates retinal progenitor cell differentiation in vitro"*
**作者**: Zhang Y, et al.
**摘要**: 通过体外细胞实验证明，OTX2重组蛋白可激活视网膜发育相关基因(如CRX)的表达，提示其在视网膜细胞分化和眼部疾病治疗中的潜在作用。
3. **文献名称**: *"Structural analysis of OTX2 homeodomain using recombinant protein crystallography"*
**作者**: Lee J, et al.
**摘要**: 利用X射线晶体学解析了OTX2重组蛋白的同源结构域三维结构，揭示了其与DNA结合的分子机制，为设计靶向OTX2的小分子药物奠定基础。
4. **文献名称**: *"OTX2 recombinant protein suppresses tumor growth in a medulloblastoma mouse model"*
**作者**: García-Ramos C, et al.
**摘要**: 在小鼠髓母细胞瘤模型中，OTX2重组蛋白通过抑制Wnt/β-catenin信号通路显著减少肿瘤体积，表明其作为新型治疗策略的可能性。
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**注意**：以上为模拟示例，实际文献需通过PubMed、Web of Science或Google Scholar等平台检索关键词“OTX2 recombinant protein”获取。建议结合研究具体方向(如疾病模型、结构生物学等)筛选文献。
**Background of OTX2 Recombinant Protein**
OTX2 (Orthodenticle Homeobox 2) is a transcription factor encoded by the *OTX2* gene, playing a critical role in embryonic development, particularly in the formation of the brain, eyes, and pituitary gland. It belongs to the bicoid class of homeodomain proteins, regulating gene expression by binding to specific DNA sequences. OTX2 is essential for the proper development of the forebrain and midbrain, as well as photoreceptor cells in the retina. Mutations or dysregulation of OTX2 are linked to severe developmental disorders, including microphthalmia, pituitary dysfunction, and neurodevelopmental impairments.
Recombinant OTX2 protein is produced using biotechnological methods, often through expression in *E. coli* or mammalian cell systems, ensuring high purity and biological activity. This engineered protein retains the functional domains of native OTX2. enabling researchers to study its role in cellular processes, gene regulation, and developmental pathways. It is widely utilized in *in vitro* and *in vivo* studies to investigate mechanisms underlying embryonic development, tissue differentiation, and disease pathogenesis.
In research, OTX2 recombinant protein aids in exploring its interaction with signaling pathways like Wnt and BMP, as well as its role in maintaining stem cell pluripotency. It also has therapeutic potential, particularly in regenerative medicine and gene therapy for congenital disorders or retinal degeneration. Additionally, OTX2 is studied in cancer biology due to its aberrant expression in tumors such as medulloblastoma and retinoblastoma.
Overall, OTX2 recombinant protein serves as a vital tool for dissecting developmental biology and disease mechanisms, offering insights into potential clinical applications.
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