| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ODAM |
| Uniprot No | A1E959 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 16-279aa |
| 氨基酸序列 | APLIPQRLMSASNSNELLLNLNNGQLLPLQLQGPLNSWIPPFSGILQQQQQAQIPGLSQFSLSALDQFAGLLPNQIPLTGEASFAQGAQAGQVDPLQLQTPPQTQPGPSHVMPYVFSFKMPQEQGQMFQYYPVYMVLPWEQPQQTVPRSPQQTRQQQYEEQIPFYAQFGYIPQLAEPAISGGQQQLAFDPQLGTAPEIAVMSTGEEIPYLQKEAINFRHDSAGVFMPSTSPKPSTTNVFTSAVDQTITPELPEEKDKTDSLREP |
| 预测分子量 | 33.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ODAM(Odontogenic Ameloblast-associated Protein)重组蛋白的3篇代表性文献及其摘要：
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1. **文献名称**: *"ODAM is a novel oncogenic protein in human cancers"*
**作者**: Park HS, et al.
**摘要**: 该研究首次报道ODAM在多种癌症(如乳腺癌、甲状腺癌)中的异常表达，并发现其重组蛋白通过调控EGFR/ERK信号通路促进肿瘤细胞增殖和迁移。实验表明ODAM可能作为癌症治疗的潜在靶点。
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2. **文献名称**: *"Structural and functional characterization of recombinant ODAM protein in enamel formation"*
**作者**: Hubbard MJ, et al.
**摘要**: 研究通过重组ODAM蛋白的体外表达和纯化，解析了其与牙釉质基质蛋白(如釉原蛋白)的相互作用机制，证实ODAM在成釉细胞分化和矿化过程中的关键作用，为牙釉质发育异常疾病提供了分子机制解释。
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3. **文献名称**: *"ODAM as a biomarker in saliva: Implications for periodontal disease and systemic inflammation"*
**作者**: Moffatt-Juria S, et al.
**摘要**: 利用重组ODAM蛋白开发了高灵敏度ELISA检测方法，发现唾液中的ODAM水平与牙周炎严重程度呈正相关，且与系统性炎症标志物(如CRP)存在关联，提示其作为非侵入性诊断工具的潜力。
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4. **文献名称**: *"Expression and purification of recombinant ODAM in a mammalian system for functional studies"*
**作者**: Feng J, et al.
**摘要**: 文章详细描述了在HEK293细胞中高效表达重组ODAM蛋白的优化流程，验证了其生物活性(如细胞黏附调控功能)，并探讨了其在组织再生研究中的应用前景。
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**注**：以上为模拟示例，实际文献需通过数据库(如PubMed、Web of Science)检索确认具体信息。如需真实文献，建议以关键词“ODAM recombinant protein”或“ODAM cancer biomarker”查询近年研究。
**Background of ODAM Recombinant Protein**
Odontogenic ameloblast-associated protein (ODAM) is a secreted, calcium-binding glycoprotein initially identified in dental tissues, particularly in ameloblasts responsible for enamel formation. It plays a critical role in biomineralization and cell adhesion during tooth development. Structurally, ODAM contains a conserved N-terminal secretory signal peptide and a C-terminal region rich in glutamic acid residues, which may mediate interactions with hydroxyapatite crystals. Beyond dentistry, ODAM is expressed in other mineralized tissues and has been implicated in broader physiological and pathological processes, including immune regulation, epithelial differentiation, and cancer progression.
Recombinant ODAM protein is engineered using heterologous expression systems (e.g., bacterial, mammalian, or insect cells) to study its molecular functions and applications. Its production typically involves codon-optimized DNA sequences cloned into expression vectors, followed by purification via affinity tags. Recombinant ODAM retains bioactivity, enabling research into its roles in cell signaling pathways (e.g., MAPK, Wnt) and extracellular matrix remodeling.
In biomedical research, ODAM recombinant protein serves as a tool to explore tissue regeneration, particularly in dental and bone repair, due to its potential to enhance mineralization. It is also investigated as a biomarker in diseases like periodontitis, breast cancer, and gastric cancer, where ODAM expression correlates with clinical outcomes. Additionally, its immunomodulatory properties are studied in inflammatory conditions.
Overall, ODAM recombinant protein bridges basic science and translational applications, offering insights into developmental biology, disease mechanisms, and therapeutic strategies for mineralization-related disorders.
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