| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NRL |
| Uniprot No | P54845 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-237aa |
| 氨基酸序列 | MALPPSPLAMEYVNDFDLMKFEVKREPSEGRPGPPTASLGSTPYSSVPPSPTFSEPGMVGATEGTRPGLEELYWLATLQQQLGAGEALGLSPEEAMELLQGQGPVPVDGPHGYYPGSPEETGAQHVQLAERFSDAALVSMSVRELNRQLRGCGRDEALRLKQRRRTLKNRGYAQACRSKRLQQRRGLEAERARLAAQLDALRAEVARLARERDLYKARCDRLTSSGPGSGDPSHLFL |
| 预测分子量 | 33.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NRL重组蛋白的3篇参考文献示例(文献信息为模拟示例，仅供参考)：
1. **文献名称**：*NRL重组蛋白在视网膜光感受器细胞分化中的功能研究*
**作者**：Smith J, Doe R, et al.
**摘要**：研究通过体外表达NRL重组蛋白，验证其在视网膜前体细胞向光感受器细胞分化中的调控作用，揭示其与CRX等转录因子的协同机制。
2. **文献名称**：*重组NRL蛋白的纯化及在基因治疗中的应用*
**作者**：Chen L, Wang X, et al.
**摘要**：报道了一种高效表达和纯化NRL重组蛋白的方法，并探索其在视网膜退行性疾病动物模型中促进光感受器细胞再生的潜力。
3. **文献名称**：*NRL重组蛋白与DNA结合的分子机制解析*
**作者**：Kim S, Tanaka Y, et al.
**摘要**：通过X射线晶体学分析NRL重组蛋白与靶基因启动子区域的结合模式，阐明其亮氨酸拉链结构域在调控光感受器特异性基因表达中的关键作用。
注：以上文献为示例，实际研究中请通过PubMed或专业数据库(如NCBI、ScienceDirect)检索具体文献。
**Background of NRL Recombinant Protein**
The NRL (Neural Retina Leucine zipper) protein is a critical transcription factor in retinal development, primarily regulating the differentiation and maintenance of photoreceptor cells, particularly rod photoreceptors. Expressed in the retina, NRL interacts with other regulatory proteins (e.g., CRX, NR2E3) to activate rod-specific genes while suppressing cone-specific pathways. Mutations in the *NRL* gene are linked to retinal degenerative diseases, such as retinitis pigmentosa (RP), highlighting its essential role in photoreceptor health.
Recombinant NRL protein is generated via genetic engineering, often using bacterial (e.g., *E. coli*) or mammalian expression systems. This involves cloning the *NRL* gene into expression vectors, followed by protein purification using affinity chromatography. The recombinant form retains the functional domains of native NRL, including the basic motif and leucine zipper, enabling studies on its DNA-binding activity and interactions.
Research applications include elucidating molecular mechanisms of retinal development, modeling disease pathways, and screening therapeutic candidates. For example, recombinant NRL aids in studying how mutations disrupt photoreceptor differentiation, potentially informing gene therapies. Challenges remain in mimicking post-translational modifications critical for NRL’s *in vivo* functionality, necessitating optimized expression systems.
Overall, NRL recombinant protein serves as a vital tool for advancing retinal biology and developing treatments for vision disorders.
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