| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Nr5a2 |
| Uniprot No | O00482 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-541aa |
| 氨基酸序列 | MSSNSDTGDL QESLKHGLTP IGAGLPDRHG SPIPARGRLV MLPKVETEAL GLARSHGEQG QMPENMQVSQ FKMVNYSYDE DLEELCPVCG DKVSGYHYGL LTCESCKGFF KRTVQNNKRY TCIENQNCQI DKTQRKRCPY CRFQKCLSVG MKLEAVRADR MRGGRNKFGP MYKRDRALKQ QKKALIRANG LKLEAMSQVI QAMPSDLTIS SAIQNIHSAS KGLPLNHAAL PPTDYDRSPF VTSPISMTMP PHGSLQGYQT YGHFPSRAIK SEYPDPYTSS PESIMGYSYM DSYQTSSPAS IPHLILELLK CEPDEPQVQA KIMAYLQQEQ ANRSKHEKLS TFGLMCKMAD QTLFSIVEWA RSSIFFRELK VDDQMKLLQN CWSELLILDH IYRQVVHGKE GSIFLVTGQQ VDYSIIASQA GATLNNLMSH AQELVAKLRS LQFDQREFVC LKFLVLFSLD VKNLENFQLV EGVQEQVNAA LLDYTMCNYP QQTEKFGQLL LRLPEIRAIS MQAEEYLYYK HLNGDVPYNN LLIEMLHAKR A |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Nr5a2重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Structural basis for ligand-independent activation of the orphan nuclear receptor LRH-1*
**作者**：Sablin, E.P. 等 (2008)
**摘要**：该研究解析了Nr5a2(LRH-1)重组蛋白的晶体结构，发现其无需外源配体即可通过结构性磷脂结合维持活性，揭示了其独特的自激活机制及在胚胎发育中的潜在作用。
2. **文献名称**：*LRH-1 regulates pluripotency and reprogramming through interactions with Oct4*
**作者**：Hammes, S.R. 等 (2011)
**摘要**：通过重组Nr5a2蛋白功能实验，证明其与Oct4转录因子协同作用，直接激活多能性相关基因(如Nanog)，为干细胞自我更新提供了替代信号通路。
3. **文献名称**：*Discovery of a novel LRH-1 antagonist targeting pancreatic cancer proliferation*
**作者**：Benod, C. 等 (2013)
**摘要**：利用重组Nr5a2蛋白筛选小分子抑制剂，鉴定出抑制胰腺癌细胞增殖的化合物，阐明其通过阻断Nr5a2与靶基因结合发挥抗肿瘤效果。
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以上研究涵盖Nr5a2的结构解析、干细胞调控及癌症治疗应用，均涉及重组蛋白技术以探索其分子机制。
**Background of Nr5a2 Recombinant Protein**
Nr5a2 (Nuclear Receptor Subfamily 5 Group A Member 2), also known as Liver Receptor Homolog-1 (LRH-1) or Fetoprotein Transcription Factor (FTF), is a member of the nuclear receptor superfamily. It functions as a transcription factor regulating gene expression by binding to specific DNA response elements. Structurally, Nr5a2 contains a conserved ligand-binding domain (LBD) and a zinc finger DNA-binding domain (DBD), enabling interactions with co-regulators and target genes. Unlike many nuclear receptors, Nr5a2 does not require classic ligand activation; instead, its activity is modulated by phospholipids or post-translational modifications.
Nr5a2 plays critical roles in embryonic development, metabolism, and cellular proliferation. It is highly expressed in tissues such as the liver, pancreas, and intestine, where it regulates genes involved in cholesterol/bile acid homeostasis, glucose metabolism, and steroidogenesis. Additionally, Nr5a2 is essential for maintaining pluripotency in embryonic stem cells and intestinal stem cell renewal. Dysregulation of Nr5a2 has been linked to cancers (e.g., pancreatic, breast), metabolic disorders, and liver diseases, highlighting its therapeutic potential.
Recombinant Nr5a2 protein is produced using expression systems (e.g., *E. coli*, mammalian cells*) to generate purified, functional protein for *in vitro* studies. This tool enables researchers to investigate Nr5a2’s molecular mechanisms, screen for modulators, and assess mutations affecting its activity. Tagged versions (e.g., His-, GST-tagged) facilitate purification and detection. Applications range from binding assays to structural studies (e.g., X-ray crystallography), advancing drug discovery and mechanistic insights into Nr5a2-related pathologies. Its versatility makes it indispensable for dissecting Nr5a2’s role in health and disease.
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