| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NR2F6 |
| Uniprot No | P10588 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-404aa |
| 氨基酸序列 | MAMVTGGWGGPGGDTNGVDKAGGYPRAAEDDSASPPGAASDAEPGDEERPGLQVDCVVCGDKSSGKHYGVFTCEGCKSFFKRSIRRNLSYTCRSNRDCQIDQHHRNQCQYCRLKKCFRVGMRKEAVQRGRIPHSLPGAVAASSGSPPGSALAAVASGGDLFPGQPVSELIAQLLRAEPYPAAAGRFGAGGGAAGAVLGIDNVCELAARLLFSTVEWARHAPFFPELPVADQVALLRLSWSELFVLNAAQAALPLHTAPLLAAAGLHAAPMAAERAVAFMDQVRAFQEQVDKLGRLQVDSAEYGCLKAIALFTPDACGLSDPAHVESLQEKAQVALTEYVRAQYPSQPQRFGRLLLRLPALRAVPASLISQLFFMRLVGKTPIETLIRDMLLSGSTFNWPYGSGQ |
| 预测分子量 | 43.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NR2F6重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"The nuclear receptor NR2F6 suppresses lymphocyte activation and T helper 17 cell-mediated autoimmunity"*
**作者**：Hermann-Kleiter, N. et al.
**摘要**：本研究揭示了NR2F6重组蛋白在调节T细胞活化和自身免疫中的作用。通过体外重组表达NR2F6.发现其能够抑制IL-17等促炎因子，并减轻实验性自身免疫性脑脊髓炎(EAE)的严重程度，表明其作为免疫治疗靶点的潜力。
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2. **文献名称**：*"NR2F6 overexpression promotes hepatocellular carcinoma progression by regulating cancer stem-like properties"*
**作者**：Chen, Y. et al.
**摘要**：该研究利用重组NR2F6蛋白分析其在肝癌中的功能。实验表明，NR2F6通过激活Wnt/β-catenin信号通路增强肝癌干细胞特性，促进肿瘤侵袭和转移，提示其作为肝癌预后标志物的可能性。
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3. **文献名称**：*"Recombinant NR2F6 modulates transcriptional networks to suppress inflammatory responses in macrophages"*
**作者**：Dong, X. et al.
**摘要**：通过重组NR2F6蛋白处理巨噬细胞，研究发现其显著抑制NF-κB和AP-1介导的炎症反应，并降低TNF-α和IL-6的表达。这为NR2F6在抗炎治疗中的应用提供了分子机制支持。
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**注**：以上文献为示例性内容，实际引用时需核对具体论文的准确性。若需进一步扩展，建议通过PubMed或Google Scholar以“NR2F6 recombinant protein”为关键词检索最新研究。
**Background of NR2F6 Recombinant Protein**
NR2F6 (Nuclear Receptor Subfamily 2 Group F Member 6), also known as COUP-TFIII, is a member of the steroid/thyroid hormone nuclear receptor superfamily. It functions as a transcription factor, regulating gene expression by binding to specific DNA response elements. Structurally, NR2F6 contains a conserved DNA-binding domain (DBD) and a ligand-binding domain (LBD), though its endogenous ligand remains unidentified. Unlike other COUP-TF family members (e.g., NR2F1/2), NR2F6 exhibits distinct expression patterns and roles, particularly in immune regulation, embryonic development, and cellular differentiation.
As a transcriptional repressor, NR2F6 modulates critical pathways, including Wnt, Notch, and retinoic acid signaling, often by competing with other nuclear receptors for DNA binding or recruiting corepressors. Studies highlight its involvement in immune tolerance, where it suppresses T-cell activation and cytokine production, making it a potential therapeutic target in autoimmune diseases and cancer immunotherapy. In cancer, NR2F6 is implicated in tumor progression, metastasis, and therapy resistance, acting as both an oncogene and tumor suppressor depending on context.
Recombinant NR2F6 protein is engineered for research applications, such as studying its DNA-binding properties, protein interactions, or screening for modulators. Produced via bacterial or mammalian expression systems, it retains functional domains necessary for in vitro assays. Its availability accelerates mechanistic studies, aiding drug discovery efforts aimed at targeting NR2F6-related pathways. Despite progress, challenges remain in elucidating its ligand-dependent functions and tissue-specific roles, underscoring the need for further exploration of this multifaceted nuclear receptor.
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