| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Nkx3-2 |
| Uniprot No | P78367 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-333aa |
| 氨基酸序列 | MAVRGANTLTSFSIQAILNKKEERGGLAAPEGRPAPGGTAASVAAAPAVCCWRLFGERDAGALGGAEDSLLASPAGTRTAAGRTAESPEGWDSDSALSEENESRRRCADARGASGAGLAGGSLSLGQPVCELAASKDLEEEAAGRSDSEMSASVSGDRSPRTEDDGVGPRGAHVSALCSGAGGGGGSGPAGVAEEEEEPAAPKPRKKRSRAAFSHAQVFELERRFNHQRYLSGPERADLAASLKLTETQVKIWFQNRRYKTKRRQMAADLLASAPAAKKVAVKVLVRDDQRQYLPGEVLRPPSLLPLQPSYYYPYYCLPGWALSTCAAAAGTQ |
| 预测分子量 | 38.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Nkx3-2重组蛋白的3篇代表性文献概览：
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1. **文献名称**: *Nkx3.2/Bapx1 regulates chondrocyte maturation through bone morphogenetic protein (BMP) signaling*
**作者**: Lengner CJ et al.
**摘要**: 该研究利用重组Nkx3-2蛋白验证其作为转录抑制因子在软骨细胞分化中的作用，发现其通过调控BMP信号通路抑制过早的软骨细胞成熟，维持生长板稳态。
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2. **文献名称**: *Structural and functional analysis of the Nkx3.2 transcription factor in craniofacial development*
**作者**: Tribioli C, Lufkin T
**摘要**: 通过重组Nkx3-2蛋白的体外实验，揭示了其DNA结合结构域的关键氨基酸残基，并证明其突变会导致颅面骨骼发育异常，强调了其在胚胎模式形成中的必要性。
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3. **文献名称**: *Recombinant Nkx3.2 protein induces chondrogenic differentiation in mesenchymal stem cells*
**作者**: Kim HJ et al.
**摘要**: 研究证明重组Nkx3-2蛋白能激活Sox9等软骨相关基因，促进间充质干细胞向软骨细胞分化，为软骨再生医学提供了潜在靶点。
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*注：以上文献为示例性质，实际引用时需根据具体研究领域核实最新论文。若需精准文献，建议通过PubMed或Web of Science以“Nkx3-2 recombinant protein”为关键词检索。*
Nkx3-2 (also known as Bapx1) is a member of the NKX family of homeodomain-containing transcription factors, which play critical roles in embryonic development, particularly in skeletal patterning and organogenesis. This protein is evolutionarily conserved and functions as a transcriptional repressor, regulating gene expression by binding to specific DNA sequences through its homeodomain. During vertebrate development, Nkx3-2 is essential for the formation of axial skeletal structures, including vertebral precursors, and craniofacial bones. It is prominently expressed in mesenchymal condensations preceding chondrogenesis and osteogenesis, where it interacts with signaling pathways such as BMP and FGF to coordinate cell differentiation and tissue morphogenesis.
Recombinant Nkx3-2 protein is produced using heterologous expression systems (e.g., E. coli or mammalian cell lines) to study its biochemical properties, DNA-binding activity, and interactions with co-regulators. Researchers employ techniques like affinity chromatography and tag-based purification (e.g., His-tag, GST-tag) to isolate functional recombinant Nkx3-2. This engineered protein serves as a tool for in vitro assays, including electrophoretic mobility shift assays (EMSAs) to map DNA-binding sites, luciferase reporter assays to assess transcriptional repression, and structural studies to resolve its molecular architecture.
Studies using recombinant Nkx3-2 have advanced our understanding of skeletal dysplasia disorders, as mutations in NKX3-2 are linked to human craniofacial malformations like spondylo-megaepiphyseal-metaphyseal dysplasia. Additionally, it provides insights into regulatory networks controlling chondrocyte differentiation, offering potential therapeutic targets for bone-related pathologies. Its role in suppressing osteoblast differentiation while promoting chondrogenesis highlights its dual functionality in skeletal development.
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