| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | YNK1 |
| Uniprot No | P36010 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-153aa |
| 氨基酸序列 | MSSQTERTFIAVKPDGVQRGLVSQILSRFEKKGYKLVAIKLVKADDKLLEQHYAEHVGKPFFPKMVSFMKSGPILATVWEGKDVVRQGRTILGATNPLGSAPGTIRGDFGIDLGRNVCHGSDSVDSAEREINLWFKKEELVDWESNQAKWIYE |
| 预测分子量 | 37.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于YNK1重组蛋白的3篇虚构参考文献示例，包含文献名称、作者及摘要概括：
1. **文献名称**：*"Recombinant YNK1 Protein Expression and Its Role in Autophagy Regulation"*
**作者**：Chen L., et al.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化YNK1重组蛋白，证实其具有激酶活性。体外实验表明，YNK1通过磷酸化ATG13蛋白调控自噬起始，为探索其在神经退行性疾病中的作用提供依据。
2. **文献名称**：*"Structural Insights into YNK1 Kinase Domain and ATP-Binding Mechanism"*
**作者**：Sato K., Müller R.
**摘要**：通过X射线晶体学解析YNK1激酶结构域的三维结构，发现其ATP结合口袋的独特构象。分子动力学模拟揭示YNK1与ATP类似物的结合模式，为设计选择性抑制剂奠定基础。
3. **文献名称**：*"YNK1 Recombinant Protein Enhances Glucose Uptake in Diabetic Mouse Models"*
**作者**：Gupta S., et al.
**摘要**：利用HEK293细胞表达YNK1重组蛋白，注射至2型糖尿病模型小鼠后，显著改善肝脏胰岛素信号通路及肌肉组织葡萄糖摄取，提示其潜在代谢调控应用价值。
(注：上述文献为模拟内容，实际研究中请参考真实数据库如PubMed。)
**Background of YNK1 Recombinant Protein**
YNK1 (Yeast Nucleoside Kinase 1) is a conserved enzyme initially identified in *Saccharomyces cerevisiae*, playing a critical role in nucleotide metabolism by catalyzing the phosphorylation of nucleosides such as uridine and cytidine. This kinase activity is essential for maintaining nucleotide pools, supporting DNA/RNA synthesis, and regulating cellular energy homeostasis. YNK1 homologs exist across eukaryotes, highlighting its functional importance in cellular physiology.
The recombinant YNK1 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cells*) to enable high-purity production for biochemical and structural studies. By cloning the YNK1 gene into expression vectors, researchers produce the protein with affinity tags (e.g., His-tag) for simplified purification. Recombinant YNK1 retains enzymatic activity, allowing in vitro studies of substrate specificity, kinetic parameters, and inhibitor screening.
Interest in YNK1 stems from its potential therapeutic relevance. Dysregulated nucleotide metabolism is linked to diseases like cancer, viral infections, and mitochondrial disorders. YNK1’s role in nucleotide salvage pathways makes it a target for drug development, particularly for antivirals or chemotherapeutic agents that disrupt nucleic acid synthesis in pathogens or rapidly dividing cells. Additionally, studying recombinant YNK1 aids in understanding conserved metabolic mechanisms and evolutionary adaptations in nucleotide kinase families.
Recent advances in structural biology, such as X-ray crystallography or cryo-EM, have utilized recombinant YNK1 to resolve its 3D architecture, providing insights into active-site dynamics and guiding rational drug design. Its application extends to diagnostics, where YNK1 activity assays help profile metabolic disorders. Overall, YNK1 recombinant protein serves as a vital tool for bridging molecular biology research with translational medicine.
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