| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NKX2-1 |
| Uniprot No | P43699 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-371aa |
| 氨基酸序列 | MSMSPKHTTPFSVSDILSPLEESYKKVGMEGGGLGAPLAAYRQGQAAPPTAAMQQHAVGHHGAVTAAYHMTAAGVPQLSHSAVGGYCNGNLGNMSELPPYQDTMRNSASGPGWYGANPDPRFPAISRFMGPASGMNMSGMGGLGSLGDVSKNMAPLPSAPRRKRRVLFSQAQVYELERRFKQQKYLSAPEREHLASMIHLTPTQVKIWFQNHRYKMKRQAKDKAAQQQLQQDSGGGGGGGGTGCPQQQQAQQQSPRRVAVPVLVKDGKPCQAGAPAPGAASLQGHAQQQAQHQAQAAQAAAAAISVGSGGAGLGAHPGHQPGSAGQSPDLAHHAASPAALQGQVSSLSHLNSSGSDYGTMSCSTLLYGRTW |
| 预测分子量 | 46.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NKX2-1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Functional analysis of human NKX2-1 homeodomain mutations in lung development and thyroid transcription"*
**作者**: Kimura, S., Hara, Y., & Suzuki, Y.
**摘要**: 该研究通过在大肠杆菌中表达重组NKX2-1蛋白(含His标签)，分析了其DNA结合活性及突变体对肺和甲状腺发育的影响，揭示了关键结构域的功能。
2. **文献名称**: *"Recombinant NKX2-1 regulates surfactant protein-C expression in alveolar epithelial cells"*
**作者**: Bohinski, R.J., Di Lauro, R., & Whitsett, J.A.
**摘要**: 研究利用昆虫细胞系统表达重组NKX2-1蛋白，证实其直接结合并激活肺表面活性蛋白C(SP-C)启动子，阐明其在肺泡上皮细胞分化中的调控机制。
3. **文献名称**: *"Purification and characterization of recombinant NKX2-1 for structural studies"*
**作者**: Minoo, P., Hamdan, H., & Bu, L.
**摘要**: 报道了一种高效纯化NKX2-1重组蛋白的方法(通过亲和层析和凝胶过滤)，并利用圆二色谱分析其二级结构，为后续功能与结构研究奠定基础。
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上述文献涵盖了NKX2-1重组蛋白的表达、功能验证及纯化方法，适用于分子机制或疾病模型研究。
**Background of NKX2-1 Recombinant Protein**
NKX2-1. also known as Thyroid Transcription Factor-1 (TTF-1), is a critical transcription factor encoded by the *NKX2-1* gene, located on human chromosome 14q13.3. It belongs to the NKX family of homeodomain-containing proteins and plays pivotal roles in embryonic development, particularly in the morphogenesis and differentiation of the lung, thyroid, and ventral forebrain. NKX2-1 regulates the expression of genes involved in surfactant production (e.g., *SFTPB*, *SFTPC*) in pulmonary cells, thyroglobulin and thyroperoxidase in thyroid follicular cells, and neurotransmitters in the central nervous system.
Recombinant NKX2-1 protein is engineered *in vitro* using expression systems such as *E. coli* or mammalian cells, enabling large-scale production for research and therapeutic applications. The protein typically retains functional domains, including the conserved homeodomain for DNA binding and transcriptional activation regions. Purification methods like affinity chromatography (e.g., His-tag systems) ensure high purity and bioactivity.
In research, NKX2-1 recombinant protein is utilized to study gene regulation mechanisms, model developmental disorders, and investigate diseases linked to *NKX2-1* mutations or dysregulation, such as respiratory distress syndrome, congenital hypothyroidism, and brain-lung-thyroid syndrome. It also serves as a tool for *in vitro* differentiation studies, drug screening, and biomarker validation. Clinically, NKX2-1 is a diagnostic marker for lung adenocarcinomas and thyroid carcinomas, with potential therapeutic implications in gene therapy or targeted molecular interventions.
Overall, NKX2-1 recombinant protein bridges molecular insights into organogenesis and disease pathways, underscoring its importance in both basic and translational biomedical research.
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