| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MSH6 |
| Uniprot No | P52701 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 931-1030aa |
| 氨基酸序列 | AGFDSDYDQALADIRENEQSLLEYLEKQRNRIGCRTIVYWGIGRNRYQLE IPENFTTRNLPEEYELKSTKKGCKRYWTKTIEKKLANLINAEERRDVSLK |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MSH6重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Recombinant Human MSH6 Protein Binds to Mismatched DNA with High Affinity*
**作者**：Gupta, S., Gellert, M., Yang, W.
**摘要**：该研究利用大肠杆菌表达系统纯化重组人MSH6蛋白，发现其与MSH2形成的MutSα复合物能特异性识别单碱基错配和插入/缺失环。通过电泳迁移实验和表面等离子体共振技术，证明MSH6对错配DNA的结合亲和力显著高于其他错配修复蛋白。
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2. **文献名称**：*ATPase Activity of the MSH6-MSH2 Complex is Essential for Mismatch Repair*
**作者**：Gradia, S., Acharya, S., Fishel, R.
**摘要**：研究团队通过昆虫细胞表达系统获得重组MSH6-MSH2复合体，发现其ATP酶活性对DNA修复功能至关重要。实验显示，ATP结合位点的突变会完全抑制复合体的错配修复能力，提示ATP水解驱动修复过程的构象变化。
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3. **文献名称**：*Structural Analysis of the MSH6 Protein in DNA Damage Recognition*
**作者**：Lee, J.Y., Yang, W.
**摘要**：该文献通过X射线晶体学解析了重组MSH6蛋白的晶体结构，揭示其与DNA结合的特定结构域(如Phe-X-Glu基序)。研究还发现MSH6的N端结构域对稳定MutSα复合物与错配DNA的结合起关键作用，为设计靶向错配修复缺陷癌症的药物提供依据。
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以上文献均聚焦于重组MSH6蛋白的表达、结构及功能机制，涵盖结合特性、酶活性和结构解析等方向。
**Background of MSH6 Recombinant Protein**
MSH6 (MutS homolog 6) is a critical component of the DNA mismatch repair (MMR) system, which ensures genomic stability by correcting errors introduced during DNA replication. It belongs to the MutS family of proteins, conserved across eukaryotes, and functions as a heterodimer with MSH2. Together, the MSH2-MSH6 complex recognizes base-base mismatches and small insertion-deletion loops, initiating repair by recruiting downstream effectors like MLH1 and PMS2. Defects in MSH6 are strongly linked to Lynch syndrome, a hereditary cancer predisposition, and somatic mutations contribute to microsatellite instability (MSI) in various cancers.
Recombinant MSH6 protein is produced using engineered expression systems (e.g., *E. coli*, insect, or mammalian cells*) to enable detailed biochemical and functional studies. Its production involves cloning the *MSH6* gene into expression vectors, followed by purification via affinity tags (e.g., His-tag). Recombinant MSH6 retains key domains: an N-terminal mismatch-binding region, a connector domain, and a C-terminal ATPase domain critical for conformational changes during repair.
This protein is widely used to investigate MMR mechanisms, screen for mutations affecting repair activity, and develop diagnostic assays for MSI or Lynch syndrome. It also serves as a tool for studying interactions with chemotherapeutic agents or potential inhibitors, aiding cancer therapy research. By providing a purified, functional form of MSH6. recombinant technology advances our understanding of DNA repair pathways and their clinical implications.
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