| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | manA |
| Uniprot No | P00946 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-391aa |
| 氨基酸序列 | MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKSSSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNKHNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQPVAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIRLISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAGLTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETTISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL |
| 预测分子量 | 58.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于manA重组蛋白的3篇示例参考文献(注：以下为虚构示例，仅供格式参考)：
1. **文献名称**：*"Heterologous Expression and Characterization of a Recombinant manA-Encoded Mannanase from Bacillus licheniformis"*
**作者**：Chen, L. et al.
**摘要**：本研究在大肠杆菌中成功表达了来自Bacillus licheniformis的manA基因，纯化后的重组酶在60°C和pH 6.0时表现出最高活性，可高效降解半乳甘露聚糖，适用于工业生物质处理。
2. **文献名称**：*"Enhancement of Thermostability of Recombinant manA through Site-Directed Mutagenesis"*
**作者**：Kumar, S. & Lee, J.
**摘要**：通过定点突变对manA编码的甘露聚糖酶进行改造，突变体在75°C下的半衰期延长了3倍，结构分析表明关键氨基酸的替换增强了热稳定性。
3. **文献名称**：*"High-Yield Production of Recombinant manA in Pichia pastoris for Industrial Applications"*
**作者**：Zhang, Y. et al.
**摘要**：利用毕赤酵母表达系统优化manA的分泌表达，通过发酵条件调控使酶产量提高至2.5 g/L，并验证其在纸浆生物漂白中的应用潜力。
(提示：实际文献需通过PubMed、Web of Science等平台检索关键词“recombinant manA”或“mannanase expression”获取。)
**Background of manA Recombinant Protein**
The manA gene, encoding mannanase (mannan endo-1.4-β-mannosidase), is a critical enzyme involved in the hydrolysis of mannan polysaccharides, major components of hemicellulose in plant cell walls. Mannanases cleave β-1.4-glycosidic linkages in mannan, glucomannan, and galactomannan, releasing oligosaccharides or monosaccharides. Originally identified in microbial species like *Bacillus subtilis* and *Aspergillus niger*, manA has garnered attention for its industrial applications, including biofuel production, animal feed processing, and paper pulp bleaching.
Recombinant manA protein is produced via heterologous expression systems, such as *Escherichia coli* or yeast, to achieve high-yield, cost-effective enzyme production. Genetic engineering optimizes the gene for codon usage, promoter compatibility, and secretion signals, enhancing expression efficiency. Purification often involves affinity tags (e.g., His-tag) for streamlined downstream processing.
Structurally, manA belongs to glycoside hydrolase families GH5 or GH26. characterized by a (β/α)₈ TIM barrel fold. Catalytic residues (typically two glutamic acids) mediate acid-base hydrolysis. Engineering efforts focus on improving thermostability, pH tolerance, and substrate specificity through mutagenesis or fusion constructs.
Applications of recombinant manA span diverse sectors. In biorefineries, it aids lignocellulosic biomass degradation for bioethanol production. In agriculture, it enhances feed digestibility by breaking down antinutritional mannans. Additionally, mannanase-derived oligosaccharides serve as prebiotics in functional foods. Challenges include maintaining activity under industrial conditions and scaling production economically.
Research continues to explore novel manA variants via metagenomic mining or directed evolution, aiming to tailor enzymatic properties for emerging biotechnological needs. Its role in sustainable industries underscores manA’s importance in advancing circular bioeconomy strategies.
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