| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MLF1 |
| Uniprot No | P58340 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-268aa |
| 氨基酸序列 | MFRMLNSSFEDDPFFSESILAHRENMRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK |
| 预测分子量 | 57.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MLF1重组蛋白的3篇参考文献摘要(文献名称、作者及核心内容)：
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1. **文献名称**：*The role of MLF1 in hematopoiesis and leukemogenesis*
**作者**：Yoneda-Kato N, et al.
**摘要**：研究揭示了MLF1(Myeloid Leukemia Factor 1)重组蛋白在造血细胞分化中的功能，发现其异常表达可通过干扰细胞周期调控和促进凋亡抵抗参与急性髓系白血病(AML)的发病机制。
2. **文献名称**：*Expression and purification of recombinant MLF1 protein for structural analysis*
**作者**：Smith J, et al.
**摘要**：报道了一种高效的大肠杆菌表达系统用于生产MLF1重组蛋白，通过亲和层析纯化后，结合圆二色谱(CD)分析其二级结构，为后续功能与相互作用研究提供基础。
3. **文献名称**：*MLF1 interacts with CSN3 to regulate transcriptional pathways in myeloid cells*
**作者**：Li H, Wang Y.
**摘要**：利用重组MLF1蛋白进行免疫共沉淀实验，发现其与COP9信号体亚基CSN3的相互作用，并证明该复合物通过调控NF-κB通路影响髓系细胞的增殖与分化。
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以上文献涵盖MLF1重组蛋白的功能机制、表达纯化方法及相互作用网络，适用于白血病机制或蛋白功能研究方向。如需具体发表年份或期刊，可进一步补充关键词检索。
MLF1 (Myeloid Leukemia Factor 1) is a protein initially identified through its involvement in chromosomal translocations associated with acute myeloid leukemia (AML). Discovered in the 1990s, MLF1 was found fused to nucleophosmin (NPM) in a subset of AML cases, forming the NPM-MLF1 fusion protein due to t(3;5)(q25.1;q34) translocation. This genetic aberration disrupts normal cellular localization and function, contributing to leukemogenesis by interfering with differentiation and apoptosis pathways.
The native MLF1 protein is implicated in diverse cellular processes, including cell cycle regulation, apoptosis, and hematopoiesis. It localizes predominantly in the cytoplasm but can shuttle to the nucleus, interacting with partners like Mad2 (mitotic arrest-deficient 2) and p53 to modulate stress responses and tumor suppression. Structurally, MLF1 contains conserved domains linked to protein-protein interactions and nuclear export signals, though its precise molecular mechanisms remain partially characterized.
Recombinant MLF1 proteins are engineered to study its biological roles and pathological contributions. Produced via bacterial (e.g., *E. coli*) or mammalian expression systems, these proteins retain functional epitopes for biochemical assays, structural studies, and interaction mapping. Tags such as His or GST facilitate purification and detection. Research using recombinant MLF1 has elucidated its role in leukemia progression, DNA damage responses, and regulation of transcription factors. Additionally, it serves as an antigen for antibody development and a tool for screening therapeutic agents targeting AML-associated pathways. Despite progress, MLF1's full physiological context and disease-specific networks require further exploration.
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