| 纯度 | >80%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF2S1 |
| Uniprot No | P05198 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-315aa |
| 氨基酸序列 | MPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSE LSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCED KFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAY DAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACY GYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSV LSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLERENAEV DGDDDAEEMEAKAED |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **EIF2S1重组蛋白** 的3篇代表性文献示例(注：文献信息为模拟虚构，仅供格式参考)：
1. **文献名称**: *"Recombinant EIF2S1 expression and its role in cellular stress response"*
**作者**: Smith A, et al.
**摘要**: 本研究通过大肠杆菌表达系统成功纯化重组人源EIF2S1蛋白，并验证其在体外可被激酶PKR磷酸化，揭示了磷酸化EIF2S1对全局蛋白质翻译抑制的调控机制。
2. **文献名称**: *"Structural insights into EIF2S1 function via recombinant protein crystallography"*
**作者**: Jones B, et al.
**摘要**: 通过X射线晶体学解析了重组EIF2S1蛋白的磷酸化(Ser51)和非磷酸化状态的三维结构，阐明了其与eIF2β/γ亚基及GTP结合的分子机制。
3. **文献名称**: *"EIF2S1 mutations impair recombinant protein stability and translation initiation in neurodegenerative disease models"*
**作者**: Chen L, et al.
**摘要**: 构建了携带EIF2S1致病突变体(如R37H)的重组蛋白，证明其导致eIF2复合体组装异常，并引发神经元细胞中错误折叠蛋白的累积，与阿尔茨海默病相关。
**建议**：实际文献需通过PubMed或Google Scholar检索关键词“EIF2S1 recombinant protein”“eIF2α purification”等获取，推荐关注功能研究、结构解析或疾病模型方向的论文。
**Background of EIF2S1 Recombinant Protein**
EIF2S1. also known as eukaryotic translation initiation factor 2 subunit alpha, is a critical component of the eIF2 complex, which regulates the initiation phase of protein synthesis in eukaryotic cells. This protein plays a central role in the formation of the 43S pre-initiation complex by delivering methionyl-tRNA (Met-tRNAi) to the ribosome, a GTP-dependent process essential for translating mRNA into proteins. Under stress conditions (e.g., nutrient deprivation, viral infection, or endoplasmic reticulum stress), EIF2S1 becomes phosphorylated at serine 51 by stress-responsive kinases (such as PERK, PKR, GCN2. or HRI), converting eIF2 from a GTP-bound active form to a GDP-bound inactive state. This phosphorylation event globally suppresses protein synthesis while selectively allowing translation of stress-responsive genes, such as ATF4. to promote cellular adaptation.
Recombinant EIFS2S1 protein is produced using biotechnological methods, often through expression in bacterial, yeast, or mammalian systems followed by purification. Its recombinant form retains functional properties, enabling researchers to study translational regulation, stress response pathways, and diseases linked to eIF2 dysregulation, including neurodegenerative disorders (e.g., Alzheimer’s), cancer, and metabolic syndromes.
Additionally, EIF2S1 recombinant protein serves as a tool for drug discovery, particularly in screening compounds targeting eIF2α phosphorylation or its regulatory kinases. Its role in modulating cellular stress responses and apoptosis has also made it relevant in understanding viral pathogenesis, as many viruses exploit host translation machinery. Overall, EIF2S1 recombinant protein is a vital reagent for dissecting molecular mechanisms of translation control and developing therapeutic strategies for diseases tied to proteostasis imbalance.
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