| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LAMTOR3 |
| Uniprot No | Q9UHA4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-124aa |
| 氨基酸序列 | MADDLKRFLYKKLPSVEGLHAIVVSDRDGVPVIKVANDNAPEHALRPGFLSTFALATDQGSKLGLSKNKSIICYYNTYQVVQFNRLPLVVSFIASSSANTGLIVSLEKELAPLFEELRQVVEVS |
| 预测分子量 | 40.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LAMTOR3重组蛋白的3篇参考文献及其简要摘要：
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1. **"Structural basis for the Raptor-binding mode of the mTORC1 subunit LAMTOR3"**
*作者：Filipek et al. (2018)*
摘要：通过重组LAMTOR3蛋白的结晶和结构分析，揭示了其与mTORC1复合体中Raptor蛋白的相互作用机制，阐明了其在调控细胞生长中的关键构效关系。
2. **"The Ragulator complex coordinates lysosomal membrane dynamics with nutrient signaling via LAMTOR3"**
*作者：Bar-Peled et al. (2012)*
摘要：研究利用重组LAMTOR3蛋白证明其在Ragulator复合体中的核心作用，通过调控溶酶体膜定位和Rag GTPase活性，影响mTORC1对氨基酸信号的响应。
3. **"Recombinant LAMTOR3 modulates ERK/MAPK signaling through scaffold formation"**
*作者：Nada et al. (2009)*
摘要：通过体外重组LAMTOR3表达实验，发现其作为支架蛋白参与ERK/MAPK信号通路的空间组织，影响细胞增殖和分化过程。
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以上研究均利用重组LAMTOR3蛋白，从结构、信号调控和分子互作角度揭示了其在细胞代谢与生长中的多功能性。如需具体文献链接或补充更多研究，可进一步说明。
LAMTOR3 (Late Endosomal/Lysosomal Adaptor, MAPK and mTOR Activator 3), also known as MP1 (MEK Partner 1) or MAP2K1IP1. is a critical component of the Ragulator complex, a multiprotein assembly that regulates lysosome-mediated nutrient sensing and signaling. This 12 kDa protein plays a pivotal role in orchestrating cellular responses to environmental cues by anchoring the mechanistic target of rapamycin complex 1 (mTORC1) to lysosomal membranes, a key step in activating mTORC1-dependent pathways controlling cell growth, autophagy, and metabolism.
Structurally, LAMTOR3 contains a conserved N-terminal domain that facilitates its interaction with other Ragulator subunits (LAMTOR1. LAMTOR4/5) and a flexible C-terminal region involved in membrane association. The Ragulator complex, through LAMTOR3's scaffolding function, acts as a platform for recruiting RAG GTPases, enabling amino acid-sensitive mTORC1 activation. This mechanism links nutrient availability to downstream processes like protein synthesis and metabolic reprogramming.
Recombinant LAMTOR3 protein is widely used to study mTOR signaling dynamics, lysosome biology, and cellular stress responses. Its production typically involves bacterial or mammalian expression systems with purification tags for functional assays, protein interaction studies, or structural analyses. Researchers employ it to investigate pathological conditions such as cancer (where mTOR hyperactivity is common), neurodegenerative disorders (linked to lysosomal dysfunction), and metabolic syndromes. Recent studies also implicate LAMTOR3 in immune cell regulation and viral pathogenesis, highlighting its broad biological relevance. As a tool, recombinant LAMTOR3 helps dissect the spatiotemporal control of nutrient signaling networks and identify therapeutic targets for mTOR-related diseases.
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