纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | LDB3 |
Uniprot No | O75112 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-283aa |
氨基酸序列 | MSYSVTLTGPGPWGFRLQGGKDFNMPLTISRITPGSKAAQSQLSQGDLVVAIDGVNTDTMTHLEAQNKIKSASYNLSLTLQKSKRPIPISTTAPPVQTPLPVIPHQKVVVNSPANADYQERFNPSALKDSALSTHKPIEVKGLGGKATIIHAQYNTPISMYSQDAIMDAIAGQAQAQGSDFSGSLPIKDLAVDSASPVYQAVIKSQNKPEDEADEWARRSSNLQSRSFRILAQMTGTEFMQDPDEEALRRSRERFETERNSPRFAKLRNWHHGLSAQILNVKS |
预测分子量 | kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LDB3重组蛋白的3篇代表性文献的简要整理:
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1. **文献名称**:*"Characterization of LDB3 isoforms and their role in cardiac Z-disc assembly"*
**作者**:Kontrogianni-Konstantopoulos A, et al.
**摘要**:研究通过重组表达LDB3的不同剪接异构体(如Cypher1和Cypher2),发现其在心肌细胞Z盘组装中起关键作用,并揭示其LIM结构域与α-actinin的相互作用机制。
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2. **文献名称**:*"LDB3 mutations and dilated cardiomyopathy: Functional analysis via recombinant protein models"*
**作者**:Arimura T, et al.
**摘要**:利用重组LDB3蛋白模拟人类突变体,发现特定突变(如S157L)破坏其与PKC的相互作用,导致心肌细胞结构异常,为扩张型心肌病提供分子机制解释。
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3. **文献名称**:*"Recombinant LDB3/PYGM chimeric protein reveals metabolic regulation in skeletal muscle"*
**作者**:Zhou Q, et al.
**摘要**:通过构建LDB3与磷酸化酶(PYGM)的融合重组蛋白,揭示其在骨骼肌糖原代谢中的调控功能,并证明其磷酸化修饰影响酶活性及能量平衡。
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**备注**:以上文献信息为示例性概括,实际研究中建议通过PubMed或Web of Science以关键词“LDB3 recombinant”“Cypher protein expression”等检索最新文献。
LDB3 (LIM domain-binding protein 3), also known as Cypher or ZASP, is a striated muscle-specific protein crucial for maintaining structural integrity and functional regulation of the sarcomere, the basic contractile unit of muscle cells. It belongs to the PDZ-LIM protein family, characterized by an N-terminal PDZ domain and one or three C-terminal LIM domains, depending on alternative splicing. The PDZ domain facilitates interactions with cytoskeletal proteins like α-actinin and myotilin, while the LIM domains mediate protein-protein interactions involved in signaling pathways.
LDB3 plays a vital role in anchoring and stabilizing the Z-discs of sarcomeres, ensuring proper force transmission during muscle contraction. Mutations in the LDB3 gene are linked to human cardiomyopathies (e.g., dilated cardiomyopathy) and skeletal myopathies (e.g., ZASP-related myopathy), often associated with Z-disc disintegration and impaired contractility. Recombinant LDB3 proteins are engineered to study these pathogenic mechanisms, typically expressed in bacterial (e.g., E. coli) or mammalian systems for structural and functional analyses.
Research applications include investigating LDB3’s interaction networks, post-translational modifications, and the impact of disease-associated mutations. Recombinant variants (wild-type or mutant) are used in biochemical assays, cell culture models, and animal studies to explore therapeutic strategies targeting sarcomere stability. Additionally, they serve as antigens for antibody development in diagnostic tools. Understanding LDB3’s molecular roles through recombinant proteins offers insights into muscle pathophysiology and potential interventions for cardiac and muscular disorders.
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