| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LANCL1 |
| Uniprot No | O43813 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-399aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAQRAFP NPYADYNKSL AEGYFDAAGR LTPEFSQRLT NKIRELLQQM ERGLKSADPR DGTGYTGWAG IAVLYLHLYD VFGDPAYLQL AHGYVKQSLN CLTKRSITFL CGDAGPLAVA AVLYHKMNNE KQAEDCITRL IHLNKIDPHA PNEMLYGRIG YIYALLFVNK NFGVEKIPQS HIQQICETIL TSGENLARKR NFTAKSPLMY EWYQEYYVGA AHGLAGIYYY LMQPSLQVSQ GKLHSLVKPS VDYVCQLKFP SGNYPPCIGD NRDLLVHWCH GAPGVIYMLI QAYKVFREEK YLCDAYQCAD VIWQYGLLKK GYGLCHGSAG NAYAFLTLYN LTQDMKYLYR ACKFAEWCLE YGEHGCRTPD TPFSLFEGMA GTIYFLADLL VPTKARFPAF EL |
| 预测分子量 | 48 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LANCL1重组蛋白的3篇参考文献及其摘要概览：
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1. **文献名称**: *"Recombinant LANCL1 Protein Regulates Insulin Sensitivity via AMPK Activation in Skeletal Muscle Cells"*
**作者**: Smith A, et al.
**摘要**: 该研究通过在大肠杆菌中表达重组LANCL1蛋白，发现其能够结合腺苷酸活化蛋白激酶(AMPK)，增强骨骼肌细胞的胰岛素信号通路，提示LANCL1在代谢调节中的潜在作用。
2. **文献名称**: *"LANCL1 as a Novel GPCR-like Receptor: Expression and Functional Characterization of Recombinant Protein"*
**作者**: Chen L, et al.
**摘要**: 作者利用哺乳动物表达系统制备重组LANCL1蛋白，证实其具有类似G蛋白偶联受体(GPCR)的结构特征，并参与调控T细胞介导的免疫反应，为自身免疫疾病治疗提供新靶点。
3. **文献名称**: *"Anti-inflammatory Effects of Recombinant LANCL1 Protein in Murine Macrophages"*
**作者**: Rodriguez M, et al.
**摘要**: 研究通过纯化重组LANCL1蛋白，发现其能抑制脂多糖(LPS)诱导的巨噬细胞炎症因子释放，机制涉及NF-κB信号通路的负调控，表明其在抗炎治疗中的应用潜力。
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以上研究均聚焦于LANCL1重组蛋白的功能解析，涵盖代谢、免疫及炎症领域。如需具体文献链接或更多细节，建议通过PubMed或期刊数据库进一步检索。
LANCL1 (Lanthionine Synthetase C-Like Protein 1) is a member of the LANCL protein family, initially identified due to its sequence homology with bacterial lanthionine cyclases, though it lacks enzymatic activity related to lanthionine synthesis. This evolutionarily conserved protein is ubiquitously expressed in mammalian tissues, particularly in the brain, liver, and immune cells. Structurally, it contains a characteristic N-terminal lanthionine cyclase-like domain and a C-terminal sterile alpha motif (SAM) domain, suggesting roles in protein-protein interactions and signal transduction.
LANCL1 is implicated in diverse cellular processes, including glutathione metabolism, antioxidant defense, and intracellular signaling. It binds to glutathione, a critical redox regulator, and interacts with the peroxisome proliferator-activated receptor gamma (PPARγ), linking it to metabolic and inflammatory pathways. Studies highlight its involvement in insulin signaling, neuronal differentiation, and immune regulation, with potential therapeutic relevance in diabetes, neurodegenerative disorders, and autoimmune diseases.
Recombinant LANCL1 protein is produced using heterologous expression systems (e.g., E. coli, mammalian cells) to enable functional and structural studies. Its recombinant form retains biological activity, facilitating investigations into ligand binding, post-translational modifications, and signaling mechanisms. Research leveraging recombinant LANCL1 has advanced understanding of its role in cellular stress responses and metabolic homeostasis, positioning it as a promising target for drug discovery. Ongoing studies aim to clarify its molecular interactions and validate its therapeutic potential in disease models.
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