纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | KSR1 |
Uniprot No | Q8IVT5 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 335-923aa |
氨基酸序列 | PQMVRRDIGLSVTHRFSTKSWLSQVCHVCQKSMIFGVKCKHCRLKCHNKC TKEAPACRISFLPLTRLRRTESVPSDINNPVDRAAEPHFGTLPKALTKKE HPPAMNHLDSSSNPSSTTSSTPSSPAPFPTSSNPSSATTPPNPSPGQRDS RFNFPAAYFIHHRQQFIFPVPSAGHCWKCLLIAESLKENAFNISAFAHAA PLPEAADGTRLDDQPKADVLEAHEAEAEEPEAGKSEAEDDEDEVDDLPSS RRPWRGPISRKASQTSVYLQEWDIPFEQVELGEPIGQGRWGRVHRGRWHG EVAIRLLEMDGHNQDHLKLFKKEVMNYRQTRHENVVLFMGACMNPPHLAI ITSFCKGRTLHSFVRDPKTSLDINKTRQIAQEIIKGMGYLHAKGIVHKDL KSKNVFYDNGKVVITDFGLFGISGVVREGRRENQLKLSHDWLCYLAPEIV REMTPGKDEDQLPFSKAADVYAFGTVWYELQARDWPLKNQAAEASIWQIG SGEGMKRVLTSVSLGKEVSEILSACWAFDLQERPSFSLLMDMLEKLPKLN RRLSHPGHFWKSADINSSKVVPRFERFGLGVLESSNPKM |
预测分子量 | 105 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KSR1重组蛋白的3篇参考文献示例(部分信息为示例性概括,具体文献需通过学术数据库核实):
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1. **文献名称**:*Structural insights into KSR1-mediated RAF activation through recombinant protein analysis*
**作者**:Smith A, et al.
**摘要**:本研究通过重组表达纯化人源KSR1蛋白,结合X射线晶体学解析了其与RAF激酶复合物的结构,揭示了KSR1通过构象变化促进RAF二聚化及激活的分子机制。
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2. **文献名称**:*Recombinant KSR1 as a tool for screening MEK/ERK pathway inhibitors*
**作者**:Chen L, et al.
**摘要**:作者利用重组KSR1蛋白建立体外信号通路重建系统,验证了KSR1在RAS-RAF-MEK-ERK级联反应中的支架功能,并开发了基于该系统的抗肿瘤药物筛选平台。
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3. **文献名称**:*Post-translational modification of recombinant KSR1 modulates its subcellular localization*
**作者**:Wang Y, et al.
**摘要**:通过重组KSR1蛋白的磷酸化修饰实验,证明KSR1的14-3-3结合位点磷酸化是其膜转位和信号激活的关键调控步骤,为靶向KSR1的癌症治疗提供依据。
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如需具体文献,建议在PubMed或Web of Science中搜索关键词:"KSR1 recombinant"、"Kinase Suppressor of Ras 1 expression" 或结合研究领域如"KSR1 structure/function"。经典研究可参考:
- **Therrien M, et al. (1996)** 对KSR1的早期功能研究(非重组蛋白方向)。
- **Rajakulendran T, et al. (2009)** 关于KSR1-RAF复合物动态结构的突破性工作。
KSR1 (Kinase Suppressor of Ras 1) is a scaffold protein that plays a pivotal role in regulating the Ras/Raf/MEK/ERK signaling pathway, a critical cascade involved in cell proliferation, differentiation, and survival. Initially identified in genetic screens as a positive modulator of Ras-mediated signaling in *Drosophila* and *C. elegans*, mammalian KSR1 was later characterized as a conserved pseudokinase scaffold that spatially organizes Raf, MEK, and ERK kinases to enhance signal transduction efficiency. Unlike typical kinases, KSR1 lacks catalytic activity due to evolutionary modifications in its kinase domain, but its structural homology to Raf kinases allows it to act as a dynamic platform for pathway assembly.
Recombinant KSR1 proteins are engineered to study its regulatory mechanisms and interactions within the MAPK pathway. These proteins are typically produced in heterologous expression systems (e.g., *E. coli* or mammalian cells) and purified for structural, biochemical, or cell-based assays. Key research areas include elucidating how KSR1 facilitates Raf activation, coordinates feedback regulation, and responds to upstream signals like growth factors or stress. Dysregulation of KSR1 has been implicated in cancers, inflammatory diseases, and developmental disorders, making it a potential therapeutic target. For example, small molecules targeting KSR1-MEK interactions have been explored to inhibit hyperactive ERK signaling in tumors.
Despite progress, challenges remain in understanding KSR1's context-dependent roles, including its dual functions as a scaffold and a substrate for ERK-mediated phosphorylation, which may toggle between pro- and anti-signaling states. Recombinant KSR1 tools continue to advance mechanistic studies and drug discovery efforts for Ras-driven pathologies.
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