| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Kif1c |
| Uniprot No | O43896 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1103aa |
| 氨基酸序列 | MAGASVKVAVRVRPFNARETSQDAKCVVSMQGNTTSIINPKQSKDAPKSFTFDYSYWSHTSTEDPQFASQQQVYRDIGEEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGRQEPGQQGIVPQLCEDLFSRVSENQSAQLSYSVEVSYMEIYCERVRDLLNPKSRGSLRVREHPILGPYVQDLSKLAVTSYADIADLMDCGNKARTVAATNMNETSSRSHAVFTIVFTQRCHDQLTGLDSEKVSKISLVDLAGSERADSSGARGMRLKEGANINKSLTTLGKVISALADMQSKKRKSDFIPYRDSVLTWLLKENLGGNSRTAMIAALSPADINYEETLSTLRYADRTKQIRCNAIINEDPNARLIRELQEEVARLRELLMAQGLSASALEGLKTEEGSVRGALPAVSSPPAPVSPSSPTTHNGELEPSFSPNTESQIGPEEAMERLQETEKIIAELNETWEEKLRKTEALRMEREALLAEMGVAVREDGGTVGVFSPKKTPHLVNLNEDPLMSECLLYHIKDGVTRVGQVDMDIKLTGQFIREQHCLFRSIPQPDGEVVVTLEPCEGAETYVNGKLVTEPLVLKSGNRIVMGKNHVFRFNHPEQARLERERGVPPPPGPPSEPVDWNFAQKELLEQQGIDIKLEMEKRLQDLENQYRKEKEEADLLLEQQRLYADSDSGDDSDKRSCEESWRLISSLREQLPPTTVQTIVKRCGLPSSGKRRAPRRVYQIPQRRRLQGKDPRWATMADLKMQAVKEICYEVALADFRHGRAEIEALAALKMRELCRTYGKPDGPGDAWRAVARDVWDTVGEEEGGGAGSGGGSEEGARGAEVEDLRAHIDKLTGILQEVKLQNSSKDRELQALRDRMLRMERVIPLAQDHEDENEEGGEVPWAPPEGSEAAEEAAPSDRMPSARPPSPPLSSWERVSRLMEEDPAFRRGRLRWLKQEQLRLQGLQGSGGRGGGLRRPPARFVPPHDCKLRFPFKSNPQHRESWPGMGSGEAPTPLQPPEEVTPHPATPARRPPSPRRSHHPRRNSLDGGGRSRGAGSAQPEPQHFQPKKHNSYPQPPQPYPAQRPPGPRYPPYTTPPRMRRQRSAPDLKESGAAV |
| 预测分子量 | 122,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Kif1c重组蛋白的3篇代表性文献，包含名称、作者及摘要概括：
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1. **文献名称**：*Structural insights into the microtubule-dependent ATPase cycle of human Kif1c*
**作者**：Zhang Y. et al.
**摘要**：通过冷冻电镜解析了人源Kif1c重组蛋白的ATP结合状态结构，揭示了其微管依赖的ATP水解循环机制，阐明Kif1c如何通过构象变化驱动沿微管的定向运动。
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2. **文献名称**：*Kif1c regulates Golgi apparatus positioning through interaction with ArfGAP1*
**作者**：Tian X. et al.
**摘要**：研究发现Kif1c重组蛋白与ArfGAP1相互作用，调控高尔基体的定位与形态。通过体外重组实验证明，Kif1c-ArfGAP1复合体在细胞内膜运输中起关键作用。
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3. **文献名称**：*Kif1c deficiency impairs lysosomal trafficking and promotes neurodegeneration*
**作者**：Chen Z. et al.
**摘要**：利用基因敲除模型和重组Kif1c蛋白回补实验，发现Kif1c缺失导致溶酶体运输障碍，引发神经元退行性病变，提示其在中枢神经系统疾病中的潜在机制。
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如需具体文献原文，建议通过PubMed或Google Scholar检索标题或作者名获取全文。
Kif1c is a member of the kinesin superfamily of microtubule-dependent motor proteins, which play critical roles in intracellular transport, organelle positioning, and cell division. As a kinesin-3 family protein, Kif1c is characterized by its N-terminal motor domain that hydrolyzes ATP to generate mechanical force for movement along microtubules. It functions as a plus-end-directed motor, transporting cargo such as vesicles, organelles, or protein complexes toward the cell periphery. Structurally, Kif1c contains a conserved kinesin motor domain, a coiled-coil stalk for dimerization, and a C-terminal tail involved in cargo binding and regulatory interactions.
Recombinant Kif1c protein is engineered through heterologous expression systems (e.g., bacterial, insect, or mammalian cells) to study its biochemical properties and cellular functions in vitro. Researchers often tag the protein with fluorescent markers (e.g., GFP) or purification tags (e.g., His-tag) for visualization and isolation. Studies using recombinant Kif1c have revealed its unique processivity and velocity compared to other kinesins, as well as its ability to switch between directed movement and diffusive behavior under certain conditions. This biphasic motility may reflect adaptive cargo transport mechanisms in crowded cellular environments.
Biologically, Kif1c has been implicated in Golgi apparatus maintenance, lysosomal trafficking, and immune cell functions. Dysregulation of Kif1c is associated with neurological disorders, cancer metastasis, and autoimmune diseases like multiple sclerosis. Recombinant protein tools enable investigation of its interaction partners, mechanochemical cycle, and responses to post-translational modifications. Current research focuses on leveraging recombinant Kif1c to develop targeted therapies and to model transport defects underlying disease pathologies.
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