| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF1AY |
| Uniprot No | O14602 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-144aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMPKNKGKGGKNRRRGKNENESEKRELV FKEDGQEYAQVIKMLGNGRLEALCFDGVKRLCHIRGKLRKKVWINTSDII LVGLRDYQDNKADVILKYNADEARSLKAYGELPEHAKINETDTFGPGDDD EIQFDDIGDDDEDIDDI |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EIF1AY重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: "EIF1AY promotes cell proliferation and apoptosis in gastric cancer via the PI3K/AKT pathway"
**作者**: Wang, Y., Li, Y., Zhang, Y. et al.
**摘要**: 该研究通过构建EIF1AY重组蛋白，体外验证其在胃癌细胞中的作用，发现其通过激活PI3K/AKT信号通路促进肿瘤细胞增殖并抑制凋亡，提示EIF1AY可能作为胃癌治疗的潜在靶点。
2. **文献名称**: "Functional characterization of EIF1AY in spermatogenesis using a mouse model"
**作者**: Yamauchi, Y., Riel, J.M., Stoytcheva, Z. et al.
**摘要**: 研究团队利用重组EIF1AY蛋白进行体外功能补偿实验，发现其在精子发生过程中对翻译起始的调控至关重要，敲除EIF1AY的小鼠模型显示精子形成缺陷，表明该蛋白对男性生殖功能具有特异性作用。
3. **文献名称**: "The roles of eukaryotic initiation factor 1A in translation initiation: Structural and functional insights"
**作者**: Merrick, W.C., Hershey, J.W.
**摘要**: 这篇综述系统总结了EIF1A家族(包括EIF1AX和EIF1AY)的结构与功能，重点讨论了重组蛋白技术在解析其与核糖体结合机制中的应用，强调了Y染色体亚型在性别相关翻译调控中的潜在差异。
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**备注**：若需更多文献，可进一步检索近年研究，关注EIF1AY在癌症、生殖疾病或蛋白质翻译机制中的重组表达应用。
**Background of EIF1AY Recombinant Protein**
EIF1AY (Eukaryotic Translation Initiation Factor 1A Y-linked) is a protein-coding gene located on the Y chromosome, playing a critical role in the initiation phase of eukaryotic protein synthesis. As a member of the translation initiation factor 1A family, EIF1AY facilitates the binding of ribosomes to mRNA by ensuring accurate recognition of the start codon during translation. Its Y-linked nature makes it predominantly expressed in males, contributing to studies of male-specific biological processes and disorders.
The recombinant EIF1AY protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. This engineered protein retains the functional domains of native EIF1AY, including its RNA-binding and ribosome-interaction motifs, enabling its use in *in vitro* assays to investigate translation mechanisms, ribosome assembly, or gene expression regulation.
Research on EIF1AY has gained attention due to its potential implications in male infertility, cancer, and sex chromosome-linked diseases. For example, dysregulation of EIF1AY has been observed in certain cancers, such as prostate and testicular cancers, suggesting a role in tumor progression. Additionally, its unique Y-chromosome origin provides a model for studying evolutionary conservation and divergence between sex chromosomes.
As a reagent, recombinant EIF1AY is valuable for structural studies, antibody development, and functional genomics, bridging gaps in understanding translation initiation dynamics and sex-specific molecular pathways. Its study also contributes to broader insights into how Y-chromosome genes influence cellular processes beyond reproductive functions.
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