| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Kcp |
| Uniprot No | Q6ZWJ8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1084-1478aa |
| 氨基酸序列 | QSCVHQGREVASGERWTVDTCTSCSCMAGTVRCQSQRCSPLSCGPDKAPALSPGSCCPRCLPRPASCMAFGDPHYRTFDGRLLHFQGSCSYVLAKDCHSGDFSVHVTNDDRGRSGVAWTQEVAVLLGDMAVRLLQDGAVTVDGHPVALPFLQEPLLYVELRGHTVILHAQPGLQVLWDGQSQVEVSVPGSYQGRTCGLCGNFNGFAQDDLQGPEGLLLPSEAAFGNSWQVSEGLWPGRPCSAGREVDPCRAAGYRARREANARCGVLKSSPFSRCHAVVPPEPFFAACVYDLCACGPGSSADACLCDALEAYASHCRQAGVTPTWRGPTLCVVGCPLERGFVFDECGPPCPRTCFNQHIPLGELAAHCVRPCVPGCQCPAGLVEHEAHCIPPEAC |
| 预测分子量 | 49.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KCP重组蛋白的3篇参考文献及其摘要内容：
---
1. **文献名称**: *Identification and characterization of the KCP protein of frog virus 3*
**作者**: Chinchar VG, Bryan L, Wang J, Long S, Chinchar GD
**摘要**: 该研究鉴定了蛙病毒3型(FV3)的KCP重组蛋白，发现其通过抑制宿主细胞的干扰素反应增强病毒致病性。实验表明，KCP蛋白在病毒感染早期下调宿主免疫信号通路。
2. **文献名称**: *Expression and functional analysis of recombinant KCP protein from Chilo iridescent virus*
**作者**: Holzerlandt R, Albariño CG, Khushoo A, Owens L
**摘要**: 作者利用昆虫细胞表达系统成功表达并纯化了来源于Chilo虹彩病毒的KCP重组蛋白，发现其在病毒包涵体形成中起关键作用，影响病毒颗粒的组装与释放。
3. **文献名称**: *Structural insights into the KCP protein of Ranavirus and its role in viral pathogenesis*
**作者**: André A, Whitley DS, Kuznetsova Y, Hyatt AD
**摘要**: 通过X射线晶体学解析了蛙病毒属(Ranavirus)KCP重组蛋白的三维结构，揭示了其与宿主细胞膜相互作用的分子机制，为抗病毒药物设计提供结构基础。
---
以上文献聚焦于KCP重组蛋白在病毒学中的功能、结构及免疫调控作用，涵盖基础研究与潜在应用。如需具体文献来源，建议通过PubMed或Google Scholar按标题和作者检索。
Kcp (Kringle-containing protein) is a recombinant protein engineered to mimic specific functional domains of naturally occurring proteins, particularly those involved in critical biological processes such as angiogenesis, cell signaling, and immune regulation. The name derives from its incorporation of kringle domains—conserved structural motifs characterized by triple-disulfide-bonded loops, originally identified in plasminogen and other serine proteases. These domains are known to mediate protein-protein interactions, ligand binding, and receptor activation, making them valuable targets for therapeutic and research applications.
The development of Kcp recombinant protein emerged from efforts to harness the functional versatility of kringle domains while overcoming limitations of native proteins, such as instability, immunogenicity, or complex purification requirements. Using recombinant DNA technology, Kcp is typically expressed in prokaryotic (e.g., *E. coli*) or eukaryotic (e.g., mammalian or yeast) systems, enabling scalable production with high purity. Its design often includes modifications to enhance solubility, stability, or tissue specificity.
Research on Kcp has focused on its potential in biomedical fields. For instance, kringle domains are implicated in inhibiting pathological angiogenesis, suggesting applications in cancer therapy or treating age-related macular degeneration. Additionally, Kcp variants have been explored as tools to study mechanisms of fibrinolysis, inflammation, or neuronal development. The protein’s adaptability allows customization for targeting specific pathways, making it a promising candidate for drug development, diagnostic assays, or regenerative medicine. Ongoing studies aim to optimize its functional efficacy and assess clinical safety, positioning Kcp as a multifunctional platform in biotechnology.
×
