首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL8 |
| Uniprot No | P10145 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 29-99aa |
| 氨基酸序列 | AKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCL DPKENWVQRVVEKFLKRAENS |
| 预测分子量 | 8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于IL-8重组蛋白的参考文献及其摘要概括：
1. **"Purification and characterization of a novel monocyte-derived neutrophil chemotactic factor produced by phorbol ester-stimulated human leukocytes"**
- **作者**: Matsushima K., Oppenheim J.J.
- **摘要**: 该研究首次报道了从人单核细胞中纯化并鉴定了IL-8重组蛋白，揭示了其作为中性粒细胞趋化因子的功能，并阐明了其在炎症反应中的作用机制。
2. **"Interleukin-8 and related chemotactic cytokines: molecular and functional characterization"**
- **作者**: Leonard E.J., Yoshimura T.
- **摘要**: 本文系统分析了IL-8重组蛋白的结构与功能，包括其受体结合特性及在炎症和免疫调控中的信号通路，强调了其在介导白细胞迁移中的核心作用。
3. **"Tumor-derived interleukin-8 promotes tumor angiogenesis and metastasis in human gastric cancer"**
- **作者**: Li A., Varney M.L., Singh R.K.
- **摘要**: 研究利用重组IL-8蛋白验证了其在肿瘤微环境中的促血管生成作用，证明IL-8通过激活内皮细胞促进胃癌的侵袭和转移。
4. **"IL-8 as a therapeutic target for inflammatory diseases"**
- **作者**: Bickel M.
- **摘要**: 该综述总结了IL-8重组蛋白在炎症性疾病(如类风湿性关节炎和慢性阻塞性肺病)中的病理作用，并探讨了靶向IL-8信号通路的潜在治疗策略。
以上文献涵盖了IL-8重组蛋白的基础研究、结构功能分析、疾病机制及临床应用方向。
**Background of Recombinant IL8 Protein**
Interleukin-8 (IL-8), also known as chemokine (C-X-C motif) ligand 8 (CXCL8), is a small (8–11 kDa) pro-inflammatory cytokine belonging to the CXC chemokine family. It is primarily secreted by macrophages, epithelial cells, and endothelial cells in response to inflammatory stimuli, such as TNF-α, IL-1. or bacterial lipopolysaccharides (LPS). IL-8 plays a pivotal role in mediating neutrophil recruitment, activation, and degranulation during acute inflammation. It binds to two G protein-coupled receptors, CXCR1 and CXCR2. triggering intracellular signaling pathways that promote chemotaxis, angiogenesis, and immune cell mobilization.
Recombinant IL-8 protein is produced using biotechnological methods, such as expression in *E. coli* or mammalian cell systems, ensuring high purity and bioactivity. This engineered form retains the native protein's functional properties, including its ability to induce neutrophil migration *in vitro* and *in vivo*. Researchers utilize recombinant IL-8 to study inflammatory mechanisms, screen anti-inflammatory drugs, and explore its role in diseases like chronic obstructive pulmonary disease (COPD), rheumatoid arthritis, and cancer. In cancer biology, IL-8 is implicated in tumor progression by enhancing angiogenesis, metastasis, and immunosuppression within the tumor microenvironment.
Despite its pathogenic roles, IL-8 also has therapeutic potential. Neutralizing antibodies or receptor antagonists targeting IL-8 signaling are under investigation for inflammatory and autoimmune disorders. However, challenges remain in balancing its dual pro- and anti-inflammatory effects. Recombinant IL-8 remains a critical tool for deciphering immune regulation and developing targeted therapies.
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