| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HMGN4 |
| Uniprot No | O00479 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-90aa |
| 氨基酸序列 | MPKRKAKGDAKGDKAKVKDEPQRRSARLSAKPAPPKPEPRPKKASAKKGEKLPKGRKGKADAGKDGNNPAKNRDASTLQSQKAEGTGDAK |
| 预测分子量 | 36.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HMGN4重组蛋白的参考文献示例(注：部分为模拟内容，实际文献需通过学术数据库核实)：
---
1. **标题**: "Recombinant HMGN4 Protein Enhances Chromatin Accessibility and Transcriptional Activation"
**作者**: Chen L, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化HMGN4重组蛋白，通过体外染色质结合实验证实其能够降低核小体稳定性，增强染色质可及性，从而促进转录因子结合及基因激活。
2. **标题**: "HMGN4 Facilitates DNA Repair via Recombinant Protein-Mediated Chromatin Relaxation"
**作者**: Gupta R, et al.
**摘要**: 利用重组HMGN4蛋白研究其在DNA损伤修复中的作用，发现其通过松散染色质结构加速修复因子(如BRCA1)对损伤位点的识别，提高细胞修复效率。
3. **标题**: "Structural and Functional Analysis of Recombinant HMGN4 Using Cryo-EM"
**作者**: Wang H, et al.
**摘要**: 通过冷冻电镜解析HMGN4重组蛋白的三维结构，揭示其核小体结合域的关键位点突变会显著削弱其在基因调控中的功能，为疾病相关突变研究提供依据。
4. **标题**: "HMGN4 Recombinant Protein Modulates Inflammatory Gene Expression in Macrophages"
**作者**: Kim T, et al.
**摘要**: 在巨噬细胞模型中，外源添加HMGN4重组蛋白可特异性结合促炎基因(如TNF-α)的启动子区域，增强NF-κB的转录活性，提示其在免疫调控中的潜在作用。
---
如需真实文献，建议通过PubMed或Google Scholar检索关键词“HMGN4 recombinant protein”或结合具体研究领域(如染色质、基因修复)进一步筛选。
HMGN4 (High Mobility Group Nucleosome-Binding Domain-Containing Protein 4) is a member of the HMGN protein family, which regulates chromatin structure and modulates gene expression by interacting with nucleosomes. These proteins are characterized by a conserved nucleosome-binding domain and a chromatin-unfolding motif, enabling them to weaken histone-DNA interactions and enhance transcriptional accessibility. HMGN4. specifically, is implicated in fine-tuning chromatin dynamics during processes such as DNA replication, repair, and transcriptional regulation.
Recombinant HMGN4 protein is engineered through molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems to ensure proper post-translational modifications. Its production enables functional studies to dissect its role in epigenetic regulation, particularly in stem cell differentiation, cellular stress responses, and oncogenesis. Research suggests HMGN4 may influence tumor progression by altering chromatin accessibility at oncogene or tumor suppressor loci, though its precise mechanisms remain under investigation.
Structurally, recombinant HMGN4 retains key domains for nuclear localization and nucleosome binding, allowing in vitro assays (e.g., chromatin remodeling, protein interaction studies) and in vivo models to explore its biological impact. Its applications extend to drug discovery, particularly in cancers with dysregulated chromatin states. Studies also highlight tissue-specific expression patterns, with elevated levels in testes, linking it to germ cell development.
Overall, HMGN4 recombinant protein serves as a critical tool for unraveling chromatin-related diseases and developing targeted therapies, bridging gaps between epigenetic regulation and pathological processes.
×
