| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HXK1 |
| Uniprot No | P19367 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 13-475aa |
| 氨基酸序列 | ELKDDQVKKIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLA |
| 预测分子量 | 55.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HXK1重组蛋白的3篇代表性文献及其摘要内容概括：
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1. **文献名称**：*Arabidopsis Hexokinase 1 Acts as a Sugar Sensor in Plant Cells*
**作者**：Jang, J.C., Leon, P., Zhou, L., Sheen, J.
**摘要**：该研究通过重组表达拟南芥HXK1蛋白，证明其在植物细胞中不仅是糖代谢酶，还直接参与葡萄糖信号传导，调控光合作用和生长发育相关基因的表达。
2. **文献名称**：*Expression and Functional Analysis of Recombinant Hexokinase 1 from Arabidopsis*
**作者**：Xiao, W., Sheen, J., Jang, J.C.
**摘要**：利用大肠杆菌系统重组表达拟南芥HXK1蛋白，通过酶活分析和突变体实验，揭示了其N端结构域在糖感知功能中的关键作用，并发现其磷酸化活性与信号传导功能可分离。
3. **文献名称**：*Biochemical Characterization of Recombinant Hexokinase 1 from Tomato*
**作者**：Moreno-Bruna, M., Baroja-Fernández, E., Muñoz, F.J.
**摘要**：从番茄中克隆HXK1基因并在原核系统中重组表达，纯化后分析其酶动力学参数，发现其对葡萄糖和果糖的催化效率差异，并探讨其在果实成熟过程中的代谢调控机制。
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以上文献均聚焦于HXK1重组蛋白的表达、功能及其在糖代谢与信号转导中的双重作用，可作为该领域研究的参考基础。
Here’s a concise yet comprehensive description of HXK1 recombinant protein:
Hexokinase 1 (HXK1) is a pivotal enzyme in glycolysis, catalyzing the ATP-dependent phosphorylation of glucose to glucose-6-phosphate, a critical step in cellular energy metabolism and sugar signaling. Originally identified in yeast and plants, HXK1 has gained attention for its dual role in metabolism and sugar-sensing pathways, particularly in regulating gene expression linked to growth, stress responses, and apoptosis. In plants, HXK1-mediated glucose signaling influences photosynthesis, senescence, and stress adaptation, making it a target for agricultural biotechnology.
Recombinant HXK1 proteins are engineered using heterologous expression systems like *E. coli* or yeast, enabling large-scale production of purified, active enzyme for research and industrial applications. These proteins retain catalytic activity and structural fidelity, allowing detailed studies of enzyme kinetics, substrate specificity, and allosteric regulation. Tagged versions (e.g., His-tagged) simplify purification via affinity chromatography.
Research applications include metabolic engineering to optimize biofuel production, investigating diabetes-related glucose dysregulation, and deciphering HXK1’s non-catalytic roles in apoptosis and disease progression. Structural studies using recombinant HXK1 have revealed conformational changes during catalysis, informing drug design for metabolic disorders. Recent work also explores its potential in CRISPR-based genome editing systems as a selectable marker. Challenges remain in maintaining post-translational modifications critical for its signaling functions when expressed in prokaryotic systems.
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