| 纯度 | > 90 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADPRH |
| Uniprot No | P54922 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-357aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMEKYVA AMVLSAAGDA LGYYNGKWEF LQDGEKIHRQ LAQLGGLDAL DVGRWRVSDD TVMHLATAEA LVEAGKAPKL TQLYYLLAKH YQDCMEDMDG RAPGGASVHN AMQLKPGKPN GWRIPFNSHE GGCGAAMRAM CIGLRFPHHS QLDTLIQVSI ESGRMTHHHP TGYLGALASA LFTAYAVNSR PPLQWGKGLM ELLPEAKKYI VQSGYFVEEN LQHWSYFQTK WENYLKLRGI LDGESAPTFP ESFGVKERDQ FYTSLSYSGW GGSSGHDAPM IAYDAVLAAG DSWKELAHRA FFHGGDSDST AAIAGCWWGV MYGFKGVSPS NYEKLEYRNR LEETARALYS LGSKEDTVIS L |
| 预测分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADPRH重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
---
1. **文献名称**：*Recombinant ADPRH Expression and Enzymatic Characterization in E. coli*
**作者**：Smith J, et al.
**摘要**：本研究通过大肠杆菌表达系统成功制备了重组ADPRH蛋白，并分析了其酶学活性。实验表明，重组ADPRH能够高效水解ADP-核糖基化修饰的底物，为研究其在细胞信号通路中的作用提供了工具。
2. **文献名称**：*Structural Insights into ADPRH via X-ray Crystallography*
**作者**：Li X, et al.
**摘要**：通过X射线晶体学解析了重组ADPRH的三维结构，揭示了其催化活性中心的关键氨基酸残基。研究为设计靶向ADPRH的小分子抑制剂奠定了基础。
3. **文献名称**：*ADPRH Knockdown and Neurodegenerative Phenotypes in Mouse Models*
**作者**：Garcia R, et al.
**摘要**：利用重组ADPRH蛋白进行体外功能补偿实验，发现ADPRH缺失会导致神经元DNA损伤积累，提示其在神经退行性疾病中的潜在保护作用。
---
**注**：以上文献为模拟示例，实际研究中请通过PubMed、Google Scholar等平台检索真实文献(关键词：ADPRH, recombinant protein, ADP-ribosylhydrolase)。
ADP-ribosylhydrolase (ADPRH), also known as ARH3. is a conserved enzyme critical for regulating post-translational protein modifications involving ADP-ribosylation. This reversible process, mediated by poly(ADP-ribose) polymerases (PARPs) and hydrolyzed by ADPRH, plays roles in DNA repair, chromatin remodeling, and cellular stress responses. Dysregulation of ADP-ribosylation is linked to neurodegenerative diseases, cancer, and inflammation, making ADPRH a key research target.
Recombinant ADPRH protein, produced via heterologous expression systems like *E. coli* or mammalian cells, enables mechanistic studies and therapeutic exploration. Its production involves cloning the ADPRH gene into expression vectors, optimizing conditions for solubility and yield, and purifying the protein using affinity chromatography. Recombinant ADPRH retains enzymatic activity, allowing *in vitro* assays to study substrate specificity, interaction partners, and inhibitors.
Research using recombinant ADPRH has clarified its role in removing ADP-ribose moieties from proteins, particularly in resolving neurotoxic accumulations linked to Parkinson’s and Alzheimer’s diseases. It also modulates PARP1-driven inflammation, suggesting therapeutic potential in autoimmune disorders. Structural studies of recombinant ADPRH have identified catalytic residues and guided drug design efforts to target ADP-ribosylation pathways.
Overall, recombinant ADPRH serves as a vital tool for deciphering ADP-ribosylation biology and developing precision therapies for related diseases. Its applications span basic enzymology, disease modeling, and high-throughput drug screening.
×
