| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Glxr |
| Uniprot No | Q9UBQ7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-328aa |
| 氨基酸序列 | MRPVRLMKVF VTRRIPAEGR VALARAADCE VEQWDSDEPI PAKELERGVA GAHGLLCLLS DHVDKRILDA AGANLKVIST MSVGIDHLAL DEIKKRGIRV GYTPDVLTDT TAELAVSLLL TTCRRLPEAI EEVKNGGWTS WKPLWLCGYG LTQSTVGIIG LGRIGQAIAR RLKPFGVQRF LYTGRQPRPE EAAEFQAEFV STPELAAQSD FIVVACSLTP ATEGLCNKDF FQKMKETAVF INISRGDVVN QDDLYQALAS GKIAAAGLDV TSPEPLPTNH PLLTLKNCVI LPHIGSATHR TRNTMSLLAA NNLLAGLRGE PMPSELKL |
| 预测分子量 | 35,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GlxR重组蛋白的3篇示例参考文献(内容基于公开研究领域知识，具体文献需通过学术数据库查询)：
1. **文献名称**：*The GlxR regulon in Corynebacterium glutamicum: A global transcriptional regulator linking carbon metabolism and energy homeostasis*
**作者**：Kohl, T.A., et al.
**摘要**：研究揭示了GlxR在谷氨酸棒状杆菌中作为全局转录因子的作用，通过重组蛋白实验证明其通过结合特定DNA序列调控糖酵解、脂肪酸代谢等相关基因的表达，参与碳源代谢与能量平衡的调控网络。
2. **文献名称**：*Characterization of the DNA-binding motif and target genes of GlxR in Corynebacterium diphtheriae*
**作者**：Schröder, J., Tauch, A.
**摘要**：通过重组GlxR蛋白的体外DNA结合实验，鉴定了其在白喉棒状杆菌中的保守结合基序(TGTCAG-N4-TGTCAG)，并筛选出多个参与中心代谢和氧化应激响应的靶基因，揭示其跨菌种的调控保守性。
3. **文献名称**：*Application of GlxR recombinant protein in metabolic engineering of amino acid-producing strains*
**作者**：Lee, J.Y., Park, S.H.
**摘要**：利用重组GlxR蛋白进行体外转录调控分析，设计代谢工程策略以解除其对赖氨酸合成途径的抑制，成功提高了工程菌株的氨基酸产量，证明其在工业生物技术中的潜在应用价值。
4. **文献名称**：*Structural insights into GlxR-mediated transcriptional regulation by X-ray crystallography*
**作者**：Kim, H.J., et al.
**摘要**：通过解析GlxR重组蛋白的晶体结构，发现其具有典型的CRP(cAMP受体蛋白)家族结构域，并揭示cAMP分子结合后引发的构象变化如何影响其与DNA的相互作用，阐明调控机制的结构基础。
**注**：以上为模拟示例，实际文献需通过PubMed/Google Scholar等平台以关键词"GlxR recombinant protein"或"GlxR transcriptional regulator"检索获取。
GlxR (Global regulator XylR-like Regulator) is a transcriptional regulatory protein primarily studied in bacterial systems, notably in Corynebacterium glutamicum and related species. Identified as a member of the CRP/FNR (cAMP receptor protein/fumarate and nitrate reductase regulator) superfamily, GlxR functions as a global regulator involved in central carbon metabolism. It binds cyclic adenosine monophosphate (cAMP) as a signaling molecule, enabling it to modulate gene expression in response to cellular energy status and nutrient availability.
Discovered during investigations into carbohydrate metabolism regulation, GlxR is structurally characterized by a conserved cAMP-binding domain and a helix-turn-helix DNA-binding motif. It typically forms homodimers to recognize specific palindromic DNA sequences upstream of target genes, repressing or activating their transcription. Key regulated pathways include glycolysis, gluconeogenesis, and the tricarboxylic acid (TCA) cycle, linking GlxR to energy homeostasis and biosynthetic processes.
Recombinant GlxR protein, produced via heterologous expression in Escherichia coli, has been instrumental in elucidating its mechanistic roles. Studies using purified GlxR revealed its cAMP-dependent conformational changes and binding affinities to promoter regions, providing insights into metabolic network coordination. Biotechnological applications leverage GlxR’s regulatory properties to engineer industrial strains for optimized metabolite production, such as amino acids or biofuels.
Research on GlxR continues to explore its interplay with other regulators and environmental signals, highlighting its potential as a metabolic engineering tool. Its conserved features across bacteria also make it a model for understanding cAMP-mediated transcriptional control in prokaryotes.
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